Phytoremediation potential of Spirulina (Arthrospira) platensis:: biosorption and toxicity studies of cadmium
Phytoremediation potential of Spirulina (Arthrospira) platensis:: biosorption and toxicity studies of cadmium
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DOI：
10.1016/s0269-7491(01)00324-4
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发表时间：
2002-01-01
影响因子：
8.9
通讯作者：
Lanza, GR
中科院分区：
文献类型：
--
作者：
Rangsayatorn, N;Upatham, ES;Lanza, GR
本研究探讨了使用钝顶螺旋藻 TISTR 8217 去除废水中低浓度镉（低于 100 mg 1）的可能性。将蓝细菌暴露于六种不同的镉浓度下 96 小时，并使用 560 nm 处的光密度测定生长速率。使用概率分析估计抑制浓度(IC50)。 24、48、72和96小时的IC50分别为13.15、16.68、17.28和18.35 mg 1 Cd。在光学显微镜和透射电子显微镜下研究细胞损伤。观察到肿胀的细胞和断裂的细丝。细胞损伤随着镉浓度的增加而增加。在暴露于接近 IC50 (14.68 mg 1) 和 IC50 (18.35 mg 1) 镉浓度的藻类中观察到超微结构变化。镉引起的改变是类囊体膜的崩解和解体、类囊体内空间的存在、多磷酸体的增加和细胞裂解。此外，还研究了藻类细胞对镉的吸附作用。发现环境因素对生物吸附有影响。镉的吸收不受溶液温度的影响，但吸附取决于 pH 值。藻细胞生物吸附的最适pH为7。镉的吸收过程很快，5分钟内完成78%的金属吸附。吸附数据很好地符合朗缪尔等温线。钝顶葡萄球菌的最大吸附容量为每克生物质 98.04 毫克镉。 (C) 2002 年，爱思唯尔科学有限公司出版。
This study examines the possibility of using Spirulina (Arthrospira) platensis TISTR 8217 to remove low concentrations of cadmium (less than 100 mg 1) from wastewater. The cyanobacteria were exposed to six different cadmium concentrations for 96 h, and the growth rate was determined using an optical density at 560 nm. The inhibiting concentration (IC50) was estimated using probit analysis. The IC50 at 24, 48, 72, and 96 h were 13.15, 16.68, 17.28, and 18.35 mg 1 Cd, respectively. Cellular damage was studied under a light microscope and a transmission electron microscope. Swollen cells and fragmented filaments were observed. Cell injury increased with increasing concentrations of cadmium. Ultrastructural changes were observed in the algae exposed to cadmium concentrations both close to IC50 (14.68 mg 1) and at IC50 (18.35 mg 1). The alterations induced by cadmium were disintegration and disorganization of the thylakoid membranes, presence of large intrathylakoidal space, increase of polyphosphate bodies, and cell lysis. In addition, the cadmium adsorption by algal cells was studied. Environmental factors were found to have an effect on biosorption. The uptake of cadmium was not affected by the temperature of the solution, but the sorption was pH dependent. The optimum pH for biosorption of algal cells was 7. The cadmium uptake process was rapid, with 78% of metal sorption completed within 5 min. The sorption data fit well to the Langmuir isotherm. The maximum adsorption capacity for S. platensis was 98.04 mg Cd per g biomass. (C) 2002 Published by Elsevier Science Ltd.