Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes
Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes
复制标题
伴清蛋白和卵清蛋白基因的体外特异性转录起始以及与腺病毒2早期和晚期基因的比较
作者：
B. Wasylyk;C. Kedinger;J. Corden;O. Brison;P. Chambon
在纯化的小牛胸腺RNA聚合酶B存在下，在无细胞系统中获得了克隆的伴清蛋白和卵清蛋白基因的特异性转录起始。与腺病毒-2早期（ElA）和主要晚期基因的比较揭示了转录效率的显著变化。伴清蛋白基因DNA的特异性片段化表明起始位点5′端的一个短DNA片段参与特异性转录。
Specific initiation of transcription of cloned conalbumin and ovalbumin genes has been obtained in a cell-free system in the presence of purified calf thymus RNA polymerase B. Comparison with the adenovirus-2 early (E1 A) and major late genes reveals marked variations in transcriptional efficiencies. Specific fragmentation of the conalbumin gene DNA indicates that a short DNA segment 5′ to the initiation site is involved in specific transcription.