Drug sensitivity test for primary culture of human cancer-cells using collagen gel embedded culture and image-analysis.
Drug sensitivity test for primary culture of human cancer-cells using collagen gel embedded culture and image-analysis.
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使用胶原凝胶嵌入培养和图像分析对人类癌细胞原代培养物进行药物敏感性测试。
DOI：
10.3892/ijo.2.6.953
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发表时间：
1993
影响因子：
5.2
通讯作者：
H. Asano
中科院分区：
文献类型：
--
作者：
M. Koezuka;N. Kondo;H. Kobayashi;S. Hara;M. Yasutomi;S. Nishida;S. Hashimoto;H. Asano
建立了一种利用胶原凝胶包埋培养和图像分析相结合的药物敏感性检测方法。在胶原凝胶中培养的人癌细胞具有极高的克隆效率和体内样药物反应。此外，首次成功利用图像分析技术，利用培养后肿瘤菌落与污染成纤维细胞的形状差异，自动区分肿瘤菌落，从而准确、方便地判断培养癌细胞的药物反应。提出了一种实际应用于人类癌细胞药敏试验的新方法。
A new drug sensitivity assay method using the collagen gel embedded culture and image analysis was developed. Human cancer cells cultured in collagen gels showed extremely high cloning efficiency and in vivo-like drug response. In addition, image analysis was used successfully for the first time to automatically discriminate cancer colonies from contaminating fibroblastic cells by taking advantage of the difference in shape between them after cultivation to determine the drug response of cultured cancer cells accurately and easily. A new approach for the practical use of the drug sensitivity test on human cancer cells is suggested.