Developmental origin of the mammalian premaxilla.
Developmental origin of the mammalian premaxilla.
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哺乳动物前颌骨的发育起源。
DOI：
10.1016/j.ydbio.2023.07.005
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发表时间：
2023
影响因子：
2.7
通讯作者：
Lan,Yu
中科院分区：
文献类型：
--
作者：
Iyyanar,PaulPR;Qin,Chuanqi;Adhikari,Nirpesh;Liu,Han;Hu,Yueh-Chiang;Jiang,Rulang;Lan,Yu
颌骨的进化在脊椎动物成功地扩展到各种各样的生态位中发挥了重要作用。在颅面生物学中，一个基本的尚未解决的问题是关于前颌骨的起源，前颌骨是所有骨的上颚中最远端的骨头。最近的研究表明，哺乳动物的前上颌骨来源于胚胎的上颌骨发育，而不是以前在鸡胚胎研究中所显示的额鼻外间充质。然而，是否哺乳动物胚胎的额鼻外胚层间充质有助于前颌骨还没有调查和工具来跟踪的额鼻外胚层间充质的贡献，在哺乳动物的面部结构是缺乏的。Alx 3基因的表达在小鼠面部形态发生开始时，在额鼻外胚层间充质中高度特异性地被激活，但在上颌间充质中不被激活。在这里，我们报告了一个新的Alx 3CreERT 2基因敲入小鼠系，表达他莫昔芬诱导的Cre DNA重组酶从theAlx 3基因座的产生和表征。在E7.5、E8.5、E9.5和E10.5，三苯氧胺处理Alx 3CreERT 2/+; Rosa 26 mTmG/+胚胎，每个胚胎诱导了内侧鼻和外侧鼻间充质的特异性标记，但不诱导上颌间充质的特异性标记。从E9.5到E16.5的Alx 3CreERT 2标记的额鼻间充质细胞的谱系追踪清楚地表明，额鼻间充质细胞产生产生前上颌骨的成骨细胞。此外，我们描述了一个Dlx 1-CreBAC转基因小鼠系，表达Cre活性在胚胎上颌，但不是额鼻间充质，并表明thedLx 1-Crelabeled胚胎上颌间充质细胞有助于上颌骨以及软组织侧的前上颌骨和上颌骨，但不是前上颌骨。这些结果清楚地表明，发育起源的前上颌骨胚胎frontonasar外胚间充质细胞在小鼠和确认的进化同源性的前上颌骨跨越sportiotes。
The evolution of jaws has played a major role in the success of vertebrate expansion into a wide variety of ecological niches. A fundamental, yet unresolved, question in craniofacial biology is about the origin of the premaxilla, the most distal bone present in the upper jaw of all amniotes. Recent reports have suggested that the mammalian premaxilla is derived from embryonic maxillary prominences rather than the frontonasal ectomesenchyme as previously shown in studies of chicken embryos. However, whether mammalian embryonic frontonasal ectomesenchyme contributes to the premaxillary bone has not been investigated and a tool to trace the contributions of the frontonasal ectomesenchyme to facial structures in mammals is lacking. The expression of theAlx3gene is activated highly specifically in the frontonasal ectomesenchyme, but not in the maxillary mesenchyme, from the beginning of facial morphogenesis in mice. Here, we report the generation and characterization of a novelAlx3CreERT2knock-in mouse line that express tamoxifen-inducible Cre DNA recombinase from theAlx3locus. Tamoxifen treatment ofAlx3CreERT2/+;Rosa26mTmG/+embryos at E7.5, E8.5, E9.5, and E10.5, each induced specific labeling of the embryonic medial nasal and lateral nasal mesenchyme but not the maxillary mesenchyme. Lineage tracing ofAlx3CreERT2-labeled frontonasal mesenchyme from E9.5 to E16.5 clearly showed that the frontonasal mesenchyme cells give rise to the osteoblasts generating the premaxillary bone. Furthermore, we characterize aDlx1-CreBAC transgenic mouse line that expresses Cre activity in the embryonic maxillary but not the frontonasal mesenchyme and show that theDlx1-Crelabeled embryonic maxillary mesenchyme cells contribute to the maxillary bone as well as the soft tissues lateral to both the premaxillary and maxillary bones but not to the premaxillary bone. These results clearly demonstrate the developmental origin of the premaxillary bone from embryonic frontonasal ectomesenchyme cells in mice and confirm the evolutionary homology of the premaxilla across amniotes.