rep-PCR fingerprinting and taxonomy based on the sequencing of the 16S rRNA gene of 54 elite commercial rhizobial strains
rep-PCR fingerprinting and taxonomy based on the sequencing of the 16S rRNA gene of 54 elite commercial rhizobial strains
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DOI：
10.1007/s00253-009-1927-6
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发表时间：
2009-07-01
影响因子：
5
通讯作者：
Hungria, Mariangela
中科院分区：
文献类型：
--
作者：
Binde, Daisy Rickli;Menna, Pamela;Hungria, Mariangela
在热带土壤中，共生固氮菌的多样性和生物技术潜力很高。然而，主要菌株的系统发育关系仍然知之甚少。此外，在巴西等国家，尽管根瘤菌接种剂得到广泛使用，但分子方法很少用于菌株分析或接种剂性能测定。在这项研究中，两个rep-PCR（BOX）指纹图谱和16 S rRNA基因的DNA序列，获得了54根瘤菌株正式授权的商业接种剂在巴西的生产。BOX-PCR已被证明是一个可靠的指纹分析工具，加强其适用性的建议，以跟踪根瘤菌菌株在文化收藏和商业接种剂的质量控制。另一方面，该方法不足以对物种甚至属进行分组或定义。9个菌株的16 S rRNA基因的核苷酸差异超过1.03%（15）与最接近的模式菌株，强烈指示新物种。这些菌株分布在伯克霍尔德菌属、根瘤菌属和慢生根瘤菌属中。
In tropical soils, diversity and biotechnological potential of symbiotic diazotrophic bacteria are high. However, the phylogenetic relationships of prominent strains are still poorly understood. In addition, in countries such as Brazil, despite the broad use of rhizobial inoculants, molecular methods are rarely used in the analysis of strains or determination of inoculant performance. In this study, both rep-PCR (BOX) fingerprintings and the DNA sequences of the 16S rRNA gene were obtained for 54 rhizobial strains officially authorized for the production of commercial inoculants in Brazil. BOX-PCR has proven to be a reliable fingerprinting tool, reinforcing the suggestion of its applicability to track rhizobial strains in culture collections and for quality control of commercial inoculants. On the other hand, the method is not adequate for grouping or defining species or even genera. Nine strains differed in more than 1.03% (15) nucleotides of the 16S rRNA gene in relation to the closest type strain, strongly indicative of new species. Those strains were distributed across the genera Burkholderia, Rhizobium, and Bradyrhizobium.