Quorum-sensing antagonist (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone influences siderophore biosynthesis in Pseudomonas putida and Pseudomonas aeruginosa
Quorum-sensing antagonist (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone influences siderophore biosynthesis in Pseudomonas putida and Pseudomonas aeruginosa
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DOI：
10.1007/s00253-004-1691-6
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发表时间：
2005-03-01
影响因子：
5
通讯作者：
Wood, TK
中科院分区：
文献类型：
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作者：
Ren, DC;Zuo, RJ;Wood, TK
恶臭假单胞菌F1的铁载体合成被发现受群体感应调节，因为在细胞进入中间指数期后，标准化的铁载体产生（每个细胞）随着细胞密度增加4.2倍;类似地，铜绿假单胞菌JB 2中的标准化铁载体浓度增加28倍，并且对于铜绿假单胞菌PAO 1观察到5.5倍增加。恶臭假单胞菌F1的群体感应和铁载体合成之间的联系的进一步证据是群体感应破坏剂（5 Z）-4-溴-5-（2-甲氧基苯基）-5（溴亚甲基）-3-丁基-2（5 H）-呋喃酮发现来自海洋红藻Delisea pulchra的（呋喃酮）以浓度依赖性方式抑制恶臭假单胞菌F1产生的铁载体的形成，其中57%的铁载体合成被100 μ g/ml呋喃酮抑制。相反，该呋喃酮在20 - 40 μ g/ml时不影响洋葱伯克霍尔德氏菌G4的铁载体合成，而在20 - 100 μ g/ml时刺激铜绿假单胞菌JB 2的铁载体合成2.5- 3.7倍。类似地，100 μ g/ml呋喃酮刺激铜绿假单胞菌PAO 1中铁载体合成约3.5倍。呋喃酮似乎与铜绿假单胞菌PAO 1的群体感应机制相互作用，因为它在铜绿假单胞菌qscR群体感应突变体中刺激较少的铁载体合成（QscR是LasI（一种酰化高丝氨酸内酯合酶）的负调节剂）。
Siderophore synthesis of Pseudomonas putida F1 was found to be regulated by quorum sensing since normalized siderophore production ( per cell) increased 4.2-fold with cell density after the cells entered middle exponential phase; similarly, normalized siderophore concentrations in Pseudomonas aeruginosa JB2 increased 28-fold, and a 5.5-fold increase was seen for P. aeruginosa PAO1. Further evidence of the link between quorum sensing and siderophore synthesis of P. putida F1 was that the quorum-sensing-disrupter (5Z)-4-bromo-5-(bromomethylene)-3- butyl-2(5H)-furanone ( furanone) from the marine red alga Delisea pulchra was found to inhibit the formation of the siderophore produced by P. putida F1 in a concentration-dependent manner, with 57% siderophore synthesis repressed by 100 mug/ml furanone. In contrast, this furanone did not affect the siderophore synthesis of Burkholderia cepacia G4 at 20 - 40 mug/ml, and stimulated siderophore synthesis of P. aeruginosa JB2 2.5- to 3.7-fold at 20 - 100 mug/ml. Similarly, 100 mug/ml furanone stimulated siderophore synthesis in P. aeruginosa PAO1 about 3.5-fold. The furanone appears to interact with the quorum-sensing machinery of P. aeruginosa PAO1 since it stimulates less siderophore synthesis in the P. aeruginosa qscR quorum-sensing mutant ( QscR is a negative regulator of LasI, an acylated homoserine lactone synthase).