Investigation of the Presence of Biofilms in Chronic Suppurative Otitis Media, Nonsuppurative Otitis Media, and Chronic Otitis Media with Cholesteatoma by Scanning Electron Microscopy
Investigation of the Presence of Biofilms in Chronic Suppurative Otitis Media, Nonsuppurative Otitis Media, and Chronic Otitis Media with Cholesteatoma by Scanning Electron Microscopy
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DOI：
10.1155/2013/638715
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Birdane, Leman
中科院分区：
文献类型：
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作者：
Kaya, Ercan;Dag, Ilknur;Birdane, Leman
Objective.生物膜在耳鼻喉科感染的发病机制中起着重要作用。然而，已经进行了非常有限的研究，以证明生物膜存在于慢性中耳炎（COM）伴或不伴中耳瘤的患者的组织中。我们的目的是研究人类慢性化脓性和非化脓性中耳炎和中耳瘤中生物膜的存在。研究设计.在手术过程中，从34名患者中总共收集了102份组织样本（中耳、乳突组织和听小骨样本）。方法.通过扫描电子显微镜（SEM）对样品进行处理以用于生物膜的研究。结果我们的研究支持生物膜参与慢性化脓性中耳炎、中耳炎和慢性非化脓性中耳炎的假说。在肥大和颗粒组织样本中的发生率高于正常粘膜。此外，与乳突和听小骨样品相比，中耳粘膜中生物膜的存在显著更高。结论在临床上，谨慎使用局部或全身抗菌药物是必不可少的，并且在手术期间，必须小心地从正常组织中去除肥大组织。
Objective. Biofilms have been shown to play a major role in the pathogenesis of otolaryngologic infections. However, very limited studies have been undertaken to demonstrate the presence of biofilms in tissues from patients with chronic otitis media (COM) with or without cholesteatoma. Our objective is to study the presence of biofilms in humans with chronic suppurative and nonsuppurative otitis media and cholesteatoma. Study Design. In all, 102 tissue specimens (middle ear, mastoid tissue, and ossicle samples) were collected during surgery from 34 patients. Methods. The samples were processed for the investigation of biofilms by scanning electron microscopy (SEM). Results. Our research supports the hypothesis in which biofilms are involved in chronic suppurative otitis media, cholesteatoma, and, to a lesser degree, chronic nonsuppurative otitis media. There were higher rates in hypertrophic and granulated tissue samples than in normal mucosa. In addition, the presence of biofilms was significantly higher in the middle ear mucosa compared with the mastoid and ossicle samples. Conclusion. In the clinic, the careful use of topical or systemic antimicrobials is essential, and, during surgery, hypertrophic tissue must be carefully removed from normal tissue.