Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. Alaska: I. Characterization of the Response.
Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. Alaska: I. Characterization of the Response.
复制标题
从黄化豌豆的切除片段中快速诱导伤口乙烯。
作者：
M. Saltveit;D. Dilley
从黄化的大麦、黄瓜、玉米、燕麦、豌豆、番茄和小麦幼苗的活跃生长区域切下的受伤组织中，乙烯合成速率发生了快速诱导的短暂增加。从7天龄黄化的豌豆的顶端附近切下完整的茎或切除9-mm的组织段，CV.阿拉斯加幼苗诱导乙烯生产的显着一致的模式。在25 ℃下，在26分钟的滞后后，从顶端钩下方9mm切除的节段的伤口诱导的乙烯产生从2.7纳升/克/小时线性增加到第一个最大值，在56分钟时为11.3纳升/克/小时。然后生产速率在90分钟时降低到最小值，在131分钟时增加到较低的第二最大值，随后在约100分钟的时间内下降到约4纳升/克/小时。去除内源性乙烯，在伤口反应开始之前，对乙烯产生的动力学没有影响。含有大量溶解乙烯的组织以指数衰减的方式释放乙烯，没有滞后期。快速诱导的伤口乙烯由组织合成，并且不仅仅是已经存在于组织中的乙烯通过新暴露的切割表面促进扩散的结果。以前受伤的根尖部分没有表现出第二次反应时，再伤。在伤口诱导的乙烯合成和CO（2）或乙烷的产生之间没有发现显著的相关性。
A rapidly induced, transitory increase in the rate of ethylene synthesis occurred in wounded tissue excised from actively growing regions of etiolated barley, cucumber, maize, oat, pea, tomato, and wheat seedlings. Cutting intact stems or excising 9-mm segments of tissue from near the apex of 7-day-old etiolated Pisum sativum L., cv. Alaska seedlings induced a remarkably consistent pattern of ethylene production. At 25 C, wound-induced ethylene production by segments excised 9 mm below the apical hook increased linearly after a lag of 26 minutes from 2.7 nanoliters per g per hour to the first maxium of 11.3 nanoliters per g per hour at 56 minutes. The rate of production then decreased to a minimum at 90 minutes, increased to a lower second maximum at 131 minutes, and subsequently declined over a period of about 100 minutes to about 4 nanoliters per g per hour. Removal of endogenous ethylene, before the wound response commenced, had no effect on the kinetics of ethylene production. Tissue containing large amounts of dissolved ethylene released it as an exponential decay with no lag period. Rapidly induced wound ethylene is synthesized by the tissue and is not merely the result of facilitated diffusion of ethylene already present in the tissue through the newly exposed cut surfaces. Previously wounded apical sections did not exhibit a second response when rewounded. No significant correlation was found between wound-induced ethylene synthesis and either CO(2) or ethane production.