Preparation of Concanavalin A-Chelating Magnetic Nanoparticles for Selective Enrichment of Glycoproteins
Preparation of Concanavalin A-Chelating Magnetic Nanoparticles for Selective Enrichment of Glycoproteins
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DOI：
10.1021/acs.analchem.5b01184
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发表时间：
2015-07-07
影响因子：
7.4
通讯作者：
Wang, Jide
中科院分区：
文献类型：
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作者：
Dong, Liping;Feng, Shun;Wang, Jide
本研究采用一种非破坏性的方法制备了刀豆球蛋白A螯合磁性纳米颗粒（Con A-MNP），并用于糖蛋白的选择性富集。首先采用一锅化学共沉淀法制备乙二胺四乙酸修饰的纳米粒子（EDTA-MNP），然后以Cu（II）阳离子为桥连基团，在EDTA-MNP上修饰ConA。所制备的吸附剂的平均直径为15 nm，表现出较强的磁响应于外加磁场。热重分析结果表明，固定化Con A含量达28wt%。对糖蛋白卵清蛋白的最大吸附容量和平衡常数分别为72.41 mg/g和0.6035 L/mg。所制备的纳米复合材料对糖蛋白表现出显着的选择性，即使摩尔比为1：600，也可以特异性地从糖蛋白和非糖蛋白的混合物中富集糖蛋白。它也被成功地应用于从真实的鸡蛋白色样品中富集糖蛋白。我们希望我们的发现将为其他人设计用于富集糖蛋白的新吸附剂提供有用的模板。
In this work, a soft and nondestructive approach was developed to prepare concanavalin A-chelating magnetic nanoparticles (Con A-MNPs) for selective enrichment of glycoproteins. Ethylenediamine tetraacetic acid-modified-MNPs (EDTA-MNPs) were prepared by a one-pot chemical coprecipitation method first, and then, Cu(II) cations were used as bridge groups to immobilize Con A on EDTA-MNPs. The as-prepared absorbents with a mean diameter of 15 nm showed a strong magnetic response to an externally applied magnetic field. The results of thermogravimetric analysis showed the content of immobilized Con A was up to 28 wt %. For glycoprotein ovalbumin, the maximum capacity and equilibrium constant were 72.41 mg/g and 0.6035 L/mg, respectively. The as-prepared nanocomposites exhibited a remarkable selectivity for glycoproteins and can enrich glycoproteins specifically from a mixture of glycoprotein and nonglycoprotein even at a molar ratio of 1:600. It was also successfully applied for the enrichment of glycoproteins from real egg white samples. We expect that our finding will serve as a helpful template for others to design new adsorbents for enriching glycoproteins.