Identification and characterization of the protein encoded by the human N-myc oncogene.
Identification and characterization of the protein encoded by the human N-myc oncogene.
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人类 N-myc 癌基因编码的蛋白质的鉴定和表征。
DOI：
10.1126/science.3008339
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Souza,LM
中科院分区：
文献类型：
--
作者：
Slamon,DJ;Boone,TC;Seeger,RC;Keith,DE;Chazin,V;Lee,HC;Souza,LM
人N-mycgene与c-mycproto-oncogene相关，并已被证明在体外具有转化潜力。许多研究报道了N-霉素对人神经母细胞瘤和视网膜母细胞瘤细胞系的扩增。在原发性肿瘤中，发现该基因的扩增与肿瘤的行为直接相关。将来自LA-N-5人神经母细胞瘤细胞的N-myc的部分互补DNA克隆的特异性限制性片段置于细菌表达载体中，以产生代表N-myc蛋白的抗原。用这些抗原免疫的兔子产生的抗血清可以识别神经母细胞瘤细胞中62-64千道尔顿的蛋白质。根据几个标准，这种蛋白质似乎是与c-mycprotein相同的原癌基因家族的一部分。此外，该蛋白片段的抗血清能够组织化学鉴定临床标本中的恶性细胞。
The human N-mycgene is related to the c-mycproto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-mycin human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the gene was found to correlate directly with behavior of the tumor. Specific restriction fragments of a partial complementary DNA clone of N-mycfrom LA-N-5 human neuroblastoma cells were placed into a bacterial expression vector for the purpose of producing antigens representative of the N-mycprotein. Rabbits immunized with these antigens produced antisera that recognized a protein of 62-64 kilodaltons in neuroblastoma cells. By several criteria, this protein appears to be part of the same proto-oncogene family as the c-mycprotein. Moreover, the antisera to fragments of this protein were capable of histochemically identifying malignant cells in clinical specimens.