Identification of HIV integration sites in infected host genomic DNA
Identification of HIV integration sites in infected host genomic DNA
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DOI：
10.1016/j.ymeth.2010.04.004
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发表时间：
2011-01-01
期刊：
影响因子：
4.8
通讯作者：
Barra, Stephen D.
中科院分区：
文献类型：
--
作者：
Ciuffi, Angela;Barra, Stephen D.
人类免疫缺陷病毒（HIV）基因信息整合到宿主基因组中是其复制和在宿主中长期存在的基础。分离和表征整合位点可用于获得数据，例如鉴定整合的特定基因组位置或理解决定HIV整合位点选择的力。本文中概述的方法描述了一种高效和精确的技术，用于使用分子克隆技术和标准测序在小规模上或使用454焦磷酸测序在大规模上鉴定宿主基因组中的HIV整合位点。(c)2010年爱思唯尔公司All rights reserved.
The integration of the Human Immunodeficiency Virus (HIV) genetic information into the host genome is fundamental for its replication and long-term persistence in the host. Isolating and characterizing the integration sites can be useful for obtaining data such as identifying the specific genomic location of integration or understanding the forces dictating HIV integration site selection. The methods outlined in this article describe a highly efficient and precise technique for identifying HIV integration sites in the host genome on a small scale using molecular cloning techniques and standard sequencing or on a massive scale using 454 pyrosequencing. (c) 2010 Elsevier Inc. All rights reserved.