Absence of canonical marks of active chromatin in developmentally regulated genes.
Absence of canonical marks of active chromatin in developmentally regulated genes.
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DOI：
10.1038/ng.3381
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发表时间：
2015-10
期刊：
影响因子：
30.8
通讯作者：
Guigó R
中科院分区：
文献类型：
--
作者：
Pérez-Lluch S;Blanco E;Tilgner H;Curado J;Ruiz-Romero M;Corominas M;Guigó R
积极的和抑制性的组蛋白修饰的相互作用被认为在基因表达的调节中起着关键作用。与这一普遍接受的观点相反，我们表明苍蝇和蠕虫发育过程中时间调控基因的转录是在缺乏典型的活性组蛋白修饰的情况下发生的。相反，强染色质标记与转录和转录后稳定性有关，我们也在哺乳动物中观察到这种联系。我们的结果支持一个模型，在该模型中，染色质标记与RNA的稳定生产有关，而未标记的染色质将允许在发育过程中快速激活和去激活基因。在这种情况下，转录因子的调节将发挥相对更重要的调节作用。
The interplay of active and repressive histone modifications is assumed to play a key role in the regulation of gene expression. In contrast to this generally accepted view, we show that transcription of genes temporally regulated during fly and worm development occurs in the absence of canonically active histone modifications. Conversely, strong chromatin marking is related to transcriptional and post-transcriptional stability, an association that we also observe in mammals. Our results support a model in which chromatin marking is associated to stable production of RNA, while unmarked chromatin would permit rapid gene activation and de-activation during development. In this case, regulation by transcription factors would play a comparatively more important regulatory role.