The isolation of a 22 kDa band after SDS-PAGE of Schistosoma mansoni adult worms and its use to demonstrate that IgE responses against the antigen(s) it contains are associated with human resistance to reinfection
The isolation of a 22 kDa band after SDS-PAGE of Schistosoma mansoni adult worms and its use to demonstrate that IgE responses against the antigen(s) it contains are associated with human resistance to reinfection
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DOI：
10.1046/j.1365-3024.1997.d01-186.x
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发表时间：
1997-02-01
影响因子：
2.2
通讯作者：
Ouma, JH
中科院分区：
文献类型：
--
作者：
Dunne, DW;Webster, M;Ouma, JH
在血吸虫病流行区，治疗后幼儿的再感染强度大于成人，高水平的寄生虫特异性IgE以年龄依赖的方式与对再感染的抵抗力相关。与不具有针对该带的IgE的个体相比，具有针对该带的IgE的个体中的曼氏再感染显著较低（Dunne等，1992）。在这里，我们报告的22 kDa的SDS-PAGE带的分离，其纳入ELISA和证明，人抗22 kDa的IgE水平有一个显着的负相关性与随后的再感染的强度。兔抗22 kDa带抗原可识别蠕虫的外被膜、肠被膜、集合管和火焰细胞。mansoni，在S. bovis和S.日本血吸虫成虫童虫和蠕虫的代谢标记证明了22 kDa抗原的体外合成和释放。
In schistosomiasis endemic areas, intensities of reinfection after treatment are greater amongst young children than amongst adults, and high levels of parasite-specific IgE are associated with resistance to reinfection in an age-dependent manner Previously we have reported that, in Western blots, a 22 kDa band was recognized by human IgE and that the incidence and intensity of S. mansoni reinfection were significantly lower amongst individuals who had IgE against this band, compared with those who did not (Dunne et al, 1992). Here we report the isolation of a 22 kDa SDS-PAGE band, its incorporation into ELISA and the demonstration that levels of human anti-22 kDa IgE had a significant negative correlation with intensities of subsequent reinfection. Rabbit anti-22 kDa band serum recognized the outer tegument, gut tegument, and the collecting ducts and flame cells of adult worms, The 22 kDa band antigen(s) was also present in 'lung'- and 'post-lung' schistosomula stages of S., mansoni, and in S, haematobium, S. bovis and S. japonicum adult worms. Metabolic labelling of schistosomula and worms demonstrated the in vitro synthesis and release of 22 kDa antigens.