Protective effects of ligustrazine on TNF-α-induced endothelial dysfunction
Protective effects of ligustrazine on TNF-α-induced endothelial dysfunction
复制标题
DOI：
10.1016/j.ejphar.2011.10.046
复制
发表时间：
2012-01-15
影响因子：
5
通讯作者：
Chen, Xian-Bing
中科院分区：
文献类型：
--
作者：
Wu, Hong-Jin;Hao, Jing;Chen, Xian-Bing
目的：观察川芎提取物川芎嗪对肿瘤坏死因子-α（TNF-α）刺激的内皮细胞的影响。在体外用TNF-α刺激人脐静脉内皮细胞（HUVEC）。一氧化氮（NO）作为内皮功能障碍的标准。以细胞内粘附分子-1（ICAM-1）和热休克蛋白60（HSP 60）作为自身免疫指标，观察川芎嗪对HUVECs的影响。川芎嗪（40 μ g/ml）显著逆转了由INF-α（5 ng/ml）诱导的HUVECs中NO产生的减少。TNF-α刺激后，ICAM-1和HSP 60表达增加，川芎嗪处理后，ICAM-1和HSP 60表达明显降低。川芎嗪通过下调ICAM-1和HSP 60的表达，增加HUVECs中NO的产生，对INF-α刺激的HUVECs具有免疫调节作用。这些结果表明，川芎嗪通过抑制免疫反应保护内皮，防止动脉粥样硬化。(C)2011 Elsevier B. V.保留所有权利。
To investigate the effects of Ligustrazine, a compound derived from chuanxiong, on tumor necrosis factor-a (TNF-alpha) stimulated endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-alpha in vitro. Nitric oxide (NO) was measured as a standard of endothelial dysfunction. Two important indicators of autoimmunity, intracellular adhesion molecular-1 (ICAM-1) and heat shock protein 60 (HSP60), were selected to evaluate the influence of Ligustrazine on HUVECs. Ligustrazine (40 mu g/ml) significantly reversed the decrease in NO production induced by INF-alpha (5 ng/ml) in HUVECs. The expressions of ICAM-1 and HSP60 were increased by TNF-alpha treatment, but dramatically inhibited by treatment with ligustrazine in TNF-alpha-stimulated cells. Ligustrazine increased the production of NO in HUVECs and had an immunomodulatory effect on HUVECs stimulated with INF-alpha by down-regulating the expression of ICAM-1 and HSP60. These results suggest that ligustrazine protects the endothelium via inhibition of immunological reactions, preventing atherosclerosis. (C) 2011 Elsevier B.V. All rights reserved.