Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors
Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors
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DOI：
10.1007/s13277-011-0162-9
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发表时间：
2011-08-01
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影响因子：
--
通讯作者：
Veselska, Renata
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文献类型：
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作者：
Krupkova, Olga, Jr.;Loja, Tomas;Veselska, Renata
巢蛋白是一种VI类中间丝蛋白，在哺乳动物中枢神经系统发育过程中的干细胞和祖细胞的细胞质中表达。在成年人中，巢蛋白仅存在于一小部分细胞和组织中，包括成年哺乳动物大脑的室下区，那里发生神经发生。巢蛋白的表达也已在诸如缺血、炎症和脑损伤的病理条件下以及在各种类型的人实体瘤及其相应的细胞系中检测到。此外，巢蛋白最近被发现存在于胶质母细胞瘤、神经母细胞瘤和血管肉瘤细胞的细胞核中，并被证明与神经母细胞瘤细胞的核DNA直接相互作用。在这里，我们进行的第一个研究巢蛋白的细胞内分布的细胞系来源于神经源性肿瘤。使用免疫检测方法，我们研究了巢蛋白表达的肿瘤来源的细胞系从11例神经母细胞瘤，髓母细胞瘤，或多形性胶质母细胞瘤。除了其标准的细胞质定位，巢蛋白存在于两个神经母细胞瘤细胞系和髓母细胞瘤细胞系的细胞核。巢蛋白只存在于细胞核中，这种蛋白质的弥漫性胞质染色，细胞核中巢蛋白阳性细胞的比例，以及染色的强度，变化。巢蛋白在细胞核中的存在被证实通过透射电子显微镜和Western印迹。我们的研究结果表明，巢蛋白在肿瘤细胞核中的存在并不是很罕见，特别是在体外条件下。
Nestin is a class VI intermediate filament protein expressed in the cytoplasm of stem and progenitor cells in the mammalian CNS during development. In adults, nestin is present only in a small subset of cells and tissues, including the subventricular zone of the adult mammalian brain, where neurogenesis occurs. Nestin expression has also been detected under such pathological conditions as ischemia, inflammation, and brain injury, as well as in various types of human solid tumors and their corresponding cell lines. Furthermore, nestin was recently found in the nuclei of glioblastoma, neuroblastoma, and angiosarcoma cells and it was proved to interact directly with the nuclear DNA in neuroblastoma cells. Here, we perform the first study of the intracellular distribution of nestin in cell lines derived from neurogenic tumors. Using immunodetection methods, we examined nestin expression in tumor-derived cell lines obtained from 11 patients with neuroblastoma, medulloblastoma, or glioblastoma multiforme. Besides its standard cytoplasmic localization, nestin was present in the nuclei of two neuroblastoma cell lines and one medulloblastoma cell line. Nestin was only present in the nuclei of cells with diffuse cytoplasmic staining for this protein, and the proportion of cells positive for nestin in nuclei, as well as the intensity of staining, varied. The presence of nestin in the nuclei was confirmed by both transmission electron microscopy and Western blotting. Our results indicate that the presence of nestin in the nuclei of tumor cells is not very rare, especially under in vitro conditions.