Differences in hormone-sensitive lipase expression in white adipose tissue from various anatomic locations of the rat.
Differences in hormone-sensitive lipase expression in white adipose tissue from various anatomic locations of the rat.
复制标题
大鼠不同解剖位置的白色脂肪组织中激素敏感性脂肪酶表达的差异。
DOI：
10.1016/0026-0495(94)90252-6
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kraemer,FB
中科院分区：
文献类型：
--
作者：
Sztalryd,C;Kraemer,FB
在大鼠中观察到分离的脂肪细胞的脂肪分解活性的区域性差异，内部脂肪储存库显示出比皮下脂肪更高的脂肪分解速率。脂肪分解的这些差异归因于多种机制，包括肾上腺素能受体、腺苷敏感性和局部血流的区域差异。在目前的研究中，我们探索了激素敏感脂肪酶(HSL)的差异是否可能导致大鼠脂肪分解的地区差异。从附睾部、腹膜后和背部皮下脂肪库分离脂肪细胞，测定HSL活性、HSL免疫反应蛋白、HSL合成率和HSL mRNA的稳态水平。HSL在附睾和腹膜后脂肪细胞中的活性相似，但这些细胞中的HSL活性是皮下脂肪细胞的两到2.5倍(P<；0.01)。HSL蛋白在附睾和腹膜后脂肪细胞中的免疫反应量也相似，但这些细胞中的HSL蛋白含量是皮下脂肪细胞的两倍(P<；.001)。在附睾部，35S-蛋氨酸掺入免疫沉淀性HSL的比率大约是皮下脂肪细胞的2.5倍(P<；.01)。最后，HSL基因在附睾库和腹膜后脂肪库中的表达水平相似，并且在这些细胞中的水平大约是皮下脂肪细胞的1.4倍(P<；.001)。这些结果表明，大鼠不同脂肪库之间基础脂解率的部位相关差异部分是由于HSL表达的差异。
Regional differences in lipolytic activity of isolated fat cells have been observed in rats, with internal fat depots displaying greater rates of lipolysis than subcutaneous fat. These differences in lipolysis have been attributed to a variety of mechanisms, including regional differences in adrenergic receptors, in adenosine sensitivity, and in regional blood flow. In the present study, we have explored whether differences in hormone-sensitive lipase (HSL), the rate-limiting enzyme in lipolysis, might contribute to regional differences in lipolysis in the rat. Adipocytes were isolated from epididymal, retroperitoneal, and dorsalsubcutaneous fat depots, and HSL activity, HSL immunoreactive protein, the rate of HSL synthesis, and the steady-state levels of HSL mRNA were assessed. HSL activity was similar in epididymal and retroperitoneal adipocytes, but was twofold to 2.5-fold greater in these cells than in subcutaneous adipocytes (P< .01). The amount of immunoreactive HSL protein was also similar in epididymal and retroperitoneal adipocytes, but was twofold greater in these cells than in subcutaneous adipocytes (P< .001). The rate of incorporation of35S-methionine into immunoprecipitable HSL was approximately 2.5-fold greater in epididymal than in subcutaneous adipocytes (P< .01). Finally, HSL mRNA levels were similar in epididymal and retroperitoneal fat depots, and were approximately 1.4-fold greater in these cells than in subcutaneous adipocytes (P< .001). These results demonstrate that site-related differences in the rate of basal lipolysis among various fat depots in the rat are in part due to variations in the expression of HSL.