Paraoxonase-2: A potential biomarker for skin cancer aggressiveness
Paraoxonase-2: A potential biomarker for skin cancer aggressiveness
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DOI：
10.1111/eci.13452
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发表时间：
2020-12-01
影响因子：
5.5
通讯作者：
Emanuelli, Monica
中科院分区：
文献类型：
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作者：
Bacchetti, Tiziana;Salvolini, Eleonora;Emanuelli, Monica
背景皮肤肿瘤包括黑色素瘤和非黑色素瘤皮肤癌。在NMSCs中，基底细胞癌(BCC)是最常见的病变。相反，尽管黑色素瘤在所有皮肤癌中所占比例不到5%，但与皮肤恶性肿瘤相关的死亡中大部分是黑色素瘤造成的。对氧磷酶-2(PON2)是一种细胞内酶，对线粒体呼吸链上产生的活性氧具有保护作用。近年来，PON2在癌症中的作用受到越来越多的关注。材料与方法采用免疫组织化学方法检测36例基底细胞癌(BCC)和29例黑色素瘤标本中PON2蛋白的表达。随后进行统计学分析，以探讨肿瘤内酶水平与临床病理特征之间是否存在相关性。结果显示，与对照组相比，PON2在BCC中过表达。特别是，在区分侵袭性较弱和侵袭性较强的肿瘤形式时，我们发现结节状基底细胞癌和对照组之间的酶水平没有显著差异。相反，浸润性基底细胞癌中PON2的表达显著高于对照组。此外，与对照相比，该酶在黑色素瘤样本中强烈上调。有趣的是，PON2的表达水平与Breslow厚度、Clark分级、退化、核分裂、淋巴结转移、原发肿瘤(PT)参数和病理分期呈正相关。结论已报道的结果提示PON2的表达水平与BCC和黑色素瘤的肿瘤侵袭性均呈正相关。
Background Cutaneous neoplasms include melanoma and non-melanoma skin cancers (NMSCs). Among NMSCs, basal cell carcinoma (BCC) represents the most common lesion. On the contrary, although accounting for less than 5% of all skin cancers, melanoma is responsible for most of cutaneous malignancy-related deaths. Paraoxonase-2 (PON2) is an intracellular enzyme exerting a protective role against production of reactive oxygen species within mitochondrial respiratory chain. Recently, a growing attention has been focused on exploring the role of PON2 in cancer. The aim of this study was to investigate the diagnostic and prognostic role of PON2 in skin neoplasms.Materials and methods 36 cases of BCC, distinguished between nodular and infiltrative lesions, as well as 29 melanoma samples were analysed by immunohistochemistry to evaluate PON2 protein expression. Subsequent statistical analyses were carried out to explore the existence of correlations between intratumour enzyme levels and clinicopathological features.Results Results obtained showed PON2 overexpression in BCCs compared with controls. In particular, distinguishing between less and more aggressive tumour forms, we found no significant differences in enzyme levels between nodular BCCs and controls. Conversely, PON2 expression was significantly higher in infiltrative BCCs compared with controls. Moreover, the enzyme was strongly upregulated in melanoma samples with respect to controls. Interestingly, PON2 levels were positively correlated with Breslow thickness, Clark level, regression, mitoses, lymph node metastases, primary tumour (pT) parameter and pathological stage.Conclusions Reported findings seem to suggest that PON2 expression levels could be positively related with tumour aggressiveness of both BCC and melanoma.