Induction of a high incidence of lung tumors in C57BL/6 mice with multiple ethyl carbamate injections
Induction of a high incidence of lung tumors in C57BL/6 mice with multiple ethyl carbamate injections
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DOI：
10.1016/s0304-3835(03)00309-4
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发表时间：
2003-08-20
期刊：
影响因子：
9.7
通讯作者：
Malkinson, AM
中科院分区：
文献类型：
--
作者：
Miller, YE;Dwyer-Nield, LD;Malkinson, AM
小鼠肺腺瘤进展为恶性肿瘤与人类肺腺癌（最常见的肺癌形式）有许多相似之处。近交系小鼠对肺肿瘤发展的易感性各不相同，诱导的遗传修饰是理解这种易感性的有力工具。许多与肺癌发病机制相关的转基因和无效突变是在高耐药的C57 BL/B6（136）背景下衍生的。由于无法可靠地诱导B6小鼠肺肿瘤限制了这些研究，我们系统地检查了几个致癌协议在B6小鼠。每周10次氨基甲酸乙酯（EC）给药导致几乎100%的肺肿瘤发生率，肿瘤多样性> 2;因此，多次EC给药是将转基因和无效突变转移到易感背景的耗时的替代方案。出版社：Elsevier爱尔兰Ltd.
Murine pulmonary adenomas progress to malignancy with many similarities to human pulmonary adenocarcinoma, the most common form of lung cancer. Inbred mice vary in their susceptibility to lung tumor development, and induced genetic modifications are a powerful tool for understanding this susceptibility. Many transgenic and null mutations relevant to lung cancer pathogenesis were derived on the highly resistant C57BL/B6 (136) background. Since the inability to reliably induce lung tumors in B6 mice limits these studies, we systematically examined several carcinogenesis protocols in B6 mice. Ten weekly ethyl carbamate (EC) doses caused a nearly 100% lung tumor incidence with a tumor multiplicity > 2; multiple EC dosing is thus an alternative to the time-consuming transfer of transgenes and null mutations to susceptible backgrounds. Published by Elsevier Ireland Ltd.