Generation of gradients having complex shapes using microfluidic networks
Generation of gradients having complex shapes using microfluidic networks
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DOI：
10.1021/ac001132d
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发表时间：
2001-03-15
影响因子：
7.4
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Dertinger, SKW;Chiu, DT;Whitesides, GM
本文介绍了在溶液中使用微流体网络具有复杂形状的梯度的产生。流动多个流体流，每个流体流携带不同浓度的物质层流地并排地产生垂直于流动方向的阶梯浓度梯度。适当设计的微通道网络用于控制物质的扩散过程，产生了一系列形状的梯度，包括线性，抛物线，皮周期性。梯度的横向尺寸范围从900到2200 μ m。本文还演示了由不同物种组成的重叠梯度的生成。由于微流体网络中的溶液以稳定状态存在并且不断更新，因此在毛细管中建立的梯度在空间和时间上是恒定的，并且可以容易地维持数小时。现在使用层流来产生梯度应该在生物和非生物研究中都是有用的。
This paper describes the generation of gradients having complex shapes in solution using microfluidic networks. Flowing multiple streams of fluid each carrying different concentrations of substances laminarly and side-by-side generated step concentration gradients perpendicular to the direction of the now. Appropriately designed networks of microchannels for controlled diffusive during of substances generated a range of shapes for the gradients, including linear, parabolic, rind periodic. The lateral dimensions of the gradients ranged from 900 to 2200 mum. This paper also demonstrates the generation of overlapping gradients composed of different species. Since solutions in the microfluidic network exist as steady states and are continuously renewed, the gradients established in the capillaries are spatially and temporally constant and can be maintained easily for periods of hours. Using laminar now to generate gradients should be useful in both biological and nonbiological research.