DNA PROBE-BASED CLASSIFICATION OF SIMULIUM-DAMNOSUM S L-BORNE AND HUMAN-DERIVED FILARIAL PARASITES IN THE ONCHOCERCIASIS CONTROL PROGRAM AREA
DNA PROBE-BASED CLASSIFICATION OF SIMULIUM-DAMNOSUM S L-BORNE AND HUMAN-DERIVED FILARIAL PARASITES IN THE ONCHOCERCIASIS CONTROL PROGRAM AREA
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DOI：
10.4269/ajtmh.1994.51.676
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发表时间：
1994-11-01
影响因子：
3.3
通讯作者：
UNNASCH, TR
中科院分区：
文献类型：
--
作者：
TOE, L;MERRIWEATHER, A;UNNASCH, TR
发展的聚合酶链反应为基础的方法，使用菌株和物种特异性的DNA探针盘尾丝虫的寄生虫的生命周期的每一个阶段允许分类的个别寄生虫。西非盘尾丝虫病控制方案已大规模应用这一技术。OCP使用DNA探针的主要目的是准确估计O.肠扭转DNA探针对OCP区幼虫的分类表明，致病性较低的O。扭转和其他丝虫寄生虫携带的Simueldamnosum s.l.导致对致盲盘尾丝虫病年传播潜力的显著高估。这种影响在OCP南部边界沿着尤为明显，在那里致盲性和致病性较低的O.扭转共存，控制区北部是动物寄生虫，特别是O. Ochengi甚至可能占主导地位。OCP应用DNA探针技术的第二个目的是确定致盲性和低致病性O。沿着控制区南部边界的受感染个体出现肠扭转。从这些研究中获得的结果基本上证实了以前流行病学研究建立的分布模式。此外，DNA探针分类表明，在致盲和致病性较低的O。肠扭转共存时，单个个体可能同时感染两种寄生虫株。
The development of polymerase chain reaction-based methods using strain- and species-specific DNA probes for Onchocerca volvulus has permitted classification of individual parasites from every stage of the parasite's life cycle. This technology has been applied on a large scale basis by Onchocerciasis Control Program (OCP) in West Africa. The primary objective of the OCP in using the DNA probes was to obtain accurate estimates of the annual transmission potential of the blinding strain of O. volvulus. The DNA probe classification of larvae collected throughout the OCP area demonstrated that larvae of less pathogenic strains of O. volvulus and other filarial parasites carried by Simulium damnosum s.l. have resulted in a significant overestimation of the annual transmission potential for blinding onchocerciasis. This effect is particularly pronounced along the southern border of the OCP, where the blinding and less pathogenic strains of O. volvulus coexist, and in the north of the control area, where animal parasites, particularly O. ochengi, may even predominate. A second objective of the OCP in applying the DNA probe technology was to determine the distribution of blinding and less pathogenic O. volvulus in infected individuals along the southern border of the control area. Results obtained from these studies have generally confirmed the distribution pattern established by previous epidemiologic studies. In addition, DNA probe classifications have demonstrated that in areas where the blinding and less pathogenic strains of O. volvulus coexist, a single individual may simultaneously be infected with both strains of the parasite.