Microfluidic Time-Division Multiplexing Accessing Resistive Pulse Sensor for Particle Analysis
Microfluidic Time-Division Multiplexing Accessing Resistive Pulse Sensor for Particle Analysis
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DOI：
10.1021/acssensors.9b01067
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发表时间：
2019-07-01
期刊：
影响因子：
8.9
通讯作者：
Guan, Weihua
中科院分区：
文献类型：
--
作者：
Choi, Gihoon;Murphy, Erica;Guan, Weihua
由于其简单性和鲁棒性，基于孔的电阻脉冲传感器已被广泛用于检测，测量和分析从纳米到微米的长度尺度的颗粒。虽然优选多个基于孔的电阻脉冲传感器来增加分析通量并克服堵塞问题，但可扩展性通常是有限的。作为回应，通过将电信领域的时分多路访问技术与微流体相结合，我们报道了一种微流体时分多路访问（TDMA）单端电阻脉冲传感器，其中颗粒可以通过可扩展数量的微流体通道进行分析。利用八通道微流控装置和聚苯乙烯颗粒作为原理验证，我们成功地证明了这种多路复用技术在测量颗粒尺寸和浓度、分析颗粒到达动力学以及区分混合种群方面是有效的。重要的是，多个感测孔的可用性提供了克服堵塞问题的鲁棒机制，即使当一些孔被堵塞时也允许分析继续。我们预计这种TDMA方法可以找到广泛的应用，并促进未来发展的多路复用电阻脉冲传感从微米尺度到纳米尺度。
Due to its simplicity and robustness, pore-based resistive pulse sensors have been widely used to detect, measure, and analyze particles at length scales ranging from nanometers to micrometers. While multiple pore-based resistive pulse sensors are preferred to increase the analysis throughput and to overcome the clogging issues, the scalability is often limited. In response, by combining the time-division multiple access technique in the telecommunication field with the microfluidics, we reported a microfluidic time-division multiplexing accessing (TDMA) single-end resistive pulse sensor, in which particles can be analyzed through a scalable number of microfluidic channels. With an eight-channel microfluidic device and polystyrene particles as proof-of-principle, we successfully demonstrated this multiplexed technology is effective in measuring the particle size and concentration, in analyzing the particle arriving dynamics, and in discriminating mixed populations. Importantly, the availability of multiple sensing pores provides a robust mechanism to overcome the clogging issue, allowing the analysis to continue even when some of the pores are clogged. We anticipate this TDMA approach could find wide applications and facilitate future development of multiplexed resistive pulse sensing from the microscale to nanoscale.