Characterization of Enzymes Catalyzing the Initial Steps of the β-Lactam Tabtoxin Biosynthesis
Characterization of Enzymes Catalyzing the Initial Steps of the β-Lactam Tabtoxin Biosynthesis
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DOI：
10.1021/acs.orglett.2c00878
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发表时间：
2022-05-05
期刊：
影响因子：
5.2
通讯作者：
Abe，Ikuro
中科院分区：
文献类型：
--
作者：
Lyu，Jiaqi;Ushimaru，Richiro;Abe，Ikuro
Tabtoxin是由植物病原性假单胞菌产生的一种含β-内酰胺环的植物毒素。在这里，我们描述了tabtoxin生物合成的早期阶段，包括由S-腺苷-L-甲硫氨酸依赖性甲基转移酶TblA催化的C-甲基化反应作为β-内酰胺构建的初始步骤。基因缺失和体外生物化学实验表明，TblD的Gcn 5相关的N-乙酰转移酶结构域催化5-甲基-1-赖氨酸的α-氨基乙酰化。早期反应步骤的建立为表征独特的β-内酰胺生物合成奠定了基础。
Tabtoxin is a β-lactam ring-containing phytotoxin produced by a plant pathogenicPseudomonasspecies. Here, we describe the early stages of tabtoxin biosynthesis, involving aC-methylation reaction catalyzed by theS-adenosyl-l-methionine-dependent methyltransferase TblA as the initial step for the β-lactam construction. Gene deletion andin vitrobiochemical assays demonstrated that the Gcn5-relatedN-acetyltransferase domain of TblD catalyzes the acetylation of the α-amino group of 5-methyl-l-lysine. This establishment of the early reaction steps lays the foundation for characterizing unique β-lactam biosynthesis.