Microbiome Alteration in Type 2 Diabetes Mellitus Model of Zebrafish
Microbiome Alteration in Type 2 Diabetes Mellitus Model of Zebrafish
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DOI：
10.1038/s41598-018-37242-x
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发表时间：
2019-01-29
影响因子：
4.6
通讯作者：
Shimada, Yasuhito
中科院分区：
文献类型：
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作者：
Okazaki, Fumiyoshi;Zang, Liqing;Shimada, Yasuhito
了解代谢紊乱（包括2型糖尿病（T2 DM））中的肠道微生物群，由于其在疾病风险和进展中的潜在作用，现在变得越来越重要。我们先前建立了T2 DM的斑马鱼模型，该模型显示葡萄糖耐受不良伴胰岛素抵抗，并对抗糖尿病药物有反应。在这项研究中，我们通过对16 S rRNA V3-V4高变区进行深度测序，分析了T2 DM斑马鱼的肠道微生物群，并使用预测宏基因组工具估算了功能谱。而对照组和T2 DM组的斑马鱼用相同种类的饲料喂养，T2 DM组的肠道菌群的多样性低于对照组。使用PICRUSt的预测宏基因组学分析揭示了T2 DM斑马鱼中KEGG通路的功能改变。在T2 DM组中，几种氨基酸代谢途径（精氨酸、脯氨酸和苯丙氨酸）下调，与先前在人体中报告的情况相似。总之，我们分析了T2 DM斑马鱼的肠道微生物组，这揭示了这些斑马鱼和T2 DM患者之间肠道细菌环境的功能相似性。T2 DM斑马鱼可以成为研究人类肥胖和相关疾病中宿主-细菌相互作用的替代模式生物。
Understanding the gut microbiota in metabolic disorders, including type 2 diabetes mellitus (T2DM), is now gaining importance due to its potential role in disease risk and progression. We previously established a zebrafish model of T2DM, which shows glucose intolerance with insulin resistance and responds to anti-diabetic drugs. In this study, we analysed the gut microbiota of T2DM zebrafish by deep sequencing the 16S rRNA V3-V4 hypervariable regions, and imputed a functional profile using predictive metagenomic tools. While control and T2DM zebrafish were fed with the same kind of feed, the gut microbiota in T2DM group was less diverse than that of the control. Predictive metagenomics profiling using PICRUSt revealed functional alternation of the KEGG pathways in T2DM zebrafish. Several amino acid metabolism pathways (arginine, proline, and phenylalanine) were downregulated in the T2DM group, similar to what has been previously reported in humans. In summary, we profiled the gut microbiome in T2DM zebrafish, which revealed functional similarities in gut bacterial environments between these zebrafish and T2DM affected humans. T2DM zebrafish can become an alternative model organism to study host-bacterial interactions in human obesity and related diseases.