The role of calcium in the stimulation of aldosterone production by adrenocorticotropin, angiotensin II, and potassium in isolated glomerulosa cells.
The role of calcium in the stimulation of aldosterone production by adrenocorticotropin, angiotensin II, and potassium in isolated glomerulosa cells.
复制标题
钙在分离肾小球细胞中促肾上腺皮质激素、血管紧张素 II 和钾刺激醛固酮产生中的作用。
作者：
J. Fakunding;Robert H. Chow;Kevin J. Catt
钙在调节醛固酮产生中的作用的分析表明，离体肾上腺细胞对ACTH、血管紧张素II和钾的类固醇生成反应高度依赖于细胞外钙浓度。生理浓度的钙需要最大的醛固酮反应的所有三个监管机构，和类固醇合成逐渐减少，降低钙浓度。ACTH刺激醛固酮和cAMP的反应，在所有的钙浓度测试，和类固醇的生产与cAMP的形成增加。细胞外钙离子浓度的减少导致ACTH浓度增加所需的半最大类固醇和cAMP的生产和醛固酮的最大输出下降。当肾小球细胞被外源性cAMP、霍乱毒素或5-羟色胺刺激时，钙离子浓度降低引起类固醇产生量下降，但不改变激动剂浓度。
Analysis of the role of calcium in the regulation of aldosterone production showed that the steroidogenic responses of isolated adrenal cells to ACTH, angiotensin II, and potassium were highly dependent on the extracellular calcium concentration. Physiological concentrations of calcium were required for maximal aldosterone responses to all three regulators, and steroidogenesis was progressively reduced by decreasing calcium concentrations. ACTH stimulated aldosterone and cAMP responses at all calcium concentrations tested, and the production of steroid was correlated with the increase in cAMP formation. Reduction of extracellular calcium concentration caused an increase in the ACTH concentration required for halfmaximal steroid and cAMP production and a decline in the maximal output of aldosterone. When zona glomerulosa cells were stimulated by exogenous cAMP, cholera toxin, or serotonin, decreased calcium concentration caused a decline in the amount of steroid produced but did not change the agonist conc...