Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles
Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles
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DOI：
10.1021/ac801046a
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发表时间：
2008-09-01
影响因子：
7.4
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Lee, Jae-Seung;Mirkin, Chad A.
我们开发了一种基于芯片的扫描测量方法用于检测汞离子（Hg 2+）。该方法利用DNA功能化的金纳米粒子的协同结合和催化性质以及He 2+的胸腺嘧啶-胸腺嘧啶错配的选择性结合。该检测方法在缓冲液和环境相关样品（湖水）中的检测限为10 nM（2 ppb）Hg 2+，这是美国环境保护署（EPA）对饮用水的[Hg 2 +]限值，比以前的比色法低1个数量级。该分析能够将Hg 2+与15种其他环境相关的金属离子区分开来。该方法是有吸引力的潜在的使用点的应用，由于其高通量，方便的读出，和便携性。
We have developed a chip-based scanometric method for the detection of mercuric ion (Hg2+). This method takes advantage of the cooperative binding and catalytic properties of DNA-functionalized gold nanoparticles and the selective binding of a thymine-thymine mismatch for He2+. The limit of detection of this assay in buffer and environmentally relevant samples (lake water) is 10 nM (2 ppb) Hg2+, which is the U.S. Environmental Protection Agency (EPA) limit of [Hg2+] for drinkable water and 1 order of magnitude lower than previous colorimetric assays. This assay is capable of discriminating Hg2+ from 15 other environmentally relevant metal ions. The method is attractive for potential point-of-use applications due to its high throughput, convenient readout, and portability.