RESOLUTION OF THE SPERM MOTILITY-STIMULATING PRINCIPLE OF FOWL SEMINAL PLASMA INTO CA-2+ AND AN UNIDENTIFIED LOW-MOLECULAR-WEIGHT FACTOR
RESOLUTION OF THE SPERM MOTILITY-STIMULATING PRINCIPLE OF FOWL SEMINAL PLASMA INTO CA-2+ AND AN UNIDENTIFIED LOW-MOLECULAR-WEIGHT FACTOR
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DOI：
10.1530/jrf.0.0810495
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发表时间：
1987-11-01
期刊：
影响因子：
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通讯作者：
WISHART, GJ
中科院分区：
文献类型：
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作者：
ASHIZAWA, K;WISHART, GJ
使用运动性的客观测定证实，家禽精浆以剂量依赖性的方式恢复和刺激家禽精子在40 ℃的运动性。用SephadexG-15分离10万g鸡精浆上清，发现有两个促运动活性峰。一个峰与钙的峰一致，并且当用Dowex 50从精浆中除去钙时不存在。另一个峰占运动刺激活性的44%，含有低分子量的可透析因子，其仍有待鉴定。
It was confirmed, using an objective assay of motility, that fowl seminal plasma restores and stimulates the motility of fowl spermatozoa at 40.degree.C in a dose-dependent manner. By separation of a 100 000 g supernatant of fowl seminal plasma with Sephadex G-15, two peaks of motility-stimulating activity were distinguished. One peak coincided with that of calcium and was absent when calicum was removed from the seminal plasma with Dowex 50. The other peak, which accounted for 44% of motility-stimulating activity, contained a low molecular weight, dialysable factor which remains to be identified.