[Analysis of the chromosome ploidy of Candida glycerinogenes].
[Analysis of the chromosome ploidy of Candida glycerinogenes].
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发表时间：
2011-03
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通讯作者：
Baoping Song;B. Zhuge;H. Fang;J. Zhuge
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作者：
Baoping Song;B. Zhuge;H. Fang;J. Zhuge
目的测定产甘油念珠菌的染色体DNA倍性。方法以酿酒酵母单倍体细胞和热带假丝酵母菌二倍体细胞为参比细胞，对产甘油念珠菌进行倍性鉴定。用二苯胺反应法测定单倍体和二倍体细胞的染色体DNA浓度。同时，用血细胞计数法分别对热带假丝酵母、甘油假丝酵母、酿酒酵母及其单倍体进行了细胞计数。由于在相同的紫外线照射条件下，单倍体细胞比二倍体细胞更容易死亡，所以我们用紫外线照射试验进行了进一步的验证。结果单细胞染色体DNA含量测定结果表明，产甘油假丝酵母为二倍体细胞。此外，酿酒酵母的单倍体细胞对紫外线的敏感性高于产甘油假丝酵母，进一步证实了这一点。结论本研究证实产甘油假丝酵母为二倍体细胞。
OBJECTIVE To determine the chromosome DNA ploidy of Candida glycerinogenes. METHODS We screened the haploid cell of Saccharomyces cerevisiae and the diploid of Candida tropicalis as the reference cell to identify the ploidy of Candida glycerinogenes. The concentrations of chromosome DNA extracted from both haploid and diploid cells were determined by the diphenylamine reaction method. Meanwhile, the cell number of Candida tropicalis, Candida glycerinogenes, Saccharomyces cerevisiae and its haploid were accounted by hemocytometer measurement method, respectively. Because in the same UV irradiation conditions,the haploid cells was more easy died than diploid cells, so we used the UV irradiation test to further verification. RESULTS The results of the content of chromosome DNA in a single cell showed that Candida glycerinogenes was a diploid cell. Moreover, it was also further confirmed because the haploid cell of Saccharomyces cerevisiae was more sensitive than Candida glycerinogenes under UV treatment. CONCLUSION By this study, we confirmed that Candida glycerinogenes was a diploid cell.