The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
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水稻碱性螺旋-环-螺旋转录因子 TDR 相互作用蛋白 2 是早期花药发育的中心开关
DOI：
10.1105/tpc.114.123745
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发表时间：
2014-04-01
期刊：
影响因子：
11.6
通讯作者：
Liang, Wanqi
中科院分区：
文献类型：
--
作者：
Fu, Zhenzhen;Yu, Jing;Liang, Wanqi
在植物雄性生殖发育中，具有短暂的干细胞样特征的分生组织样前体细胞经历细胞分裂和分化以产生花药，即雄性生殖器官。小孢子母细胞位于花药中央，由四层不同的壁包围：表皮、药室内壁、中层和绒毡层。在这里，我们报告了水稻（Oryza sativa）的基本螺旋-环-螺旋（bHLH）蛋白质TDR相互作用蛋白2（TIP 2）的功能作为一个重要的开关在分生组织的过渡和分化在早期花药发育。tip 2突变体表现为花药内三层未分化，绒毡层细胞程序性死亡中止，导致完全雄性不育。TIP 2在水稻中有两个旁系同源物，TDR和EAT 1，它们是绒毡层程序性细胞死亡的关键调节因子。我们发现TIP 2作用于TDR和EAT 1的上游，并直接调节TDR和EAT 1的表达。此外，TIP 2可以与TDR相互作用，表明TIP 2在后期花药发育中的作用。我们的研究结果表明，bHLH蛋白TIP 2，TDR和EAT 1发挥了核心作用，在调节分化，形态建成和退化的花药体细胞层，突出的作用，旁系同源bHLH蛋白在调节不同的步骤植物细胞类型的决定。
In male reproductive development in plants, meristemoid precursor cells possessing transient, stem cell-like features undergo cell divisions and differentiation to produce the anther, the male reproductive organ. The anther contains centrally positioned microsporocytes surrounded by four distinct layers of wall: the epidermis, endothecium, middle layer, and tapetum. Here, we report that the rice (Oryza sativa) basic helix-loop-helix ( bHLH) protein TDR INTERACTING PROTEIN2 (TIP2) functions as a crucial switch in the meristemoid transition and differentiation during early anther development. The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility. TIP2 has two paralogs in rice, TDR and EAT1, which are key regulators of tapetal programmed cell death. We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1. In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development. Our findings suggest that the bHLH proteins TIP2, TDR, and EAT1 play a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers, highlighting the role of paralogous bHLH proteins in regulating distinct steps of plant cell-type determination.