DETECTION OF EPSTEIN-BARR-VIRUS BY POLYMERASE CHAIN-REACTION
DETECTION OF EPSTEIN-BARR-VIRUS BY POLYMERASE CHAIN-REACTION
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DOI：
10.1128/jcm.28.10.2187-2190.1990
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发表时间：
1990-10-01
影响因子：
9.4
通讯作者：
SMITH, TF
中科院分区：
文献类型：
--
作者：
TELENTI, A;MARSHALL, WF;SMITH, TF
应用聚合酶链反应（PCR）对25例移植患者、5例传染性单核细胞增多症患者和13例健康人的血液及29例淋巴细胞增生性疾病患者的尸体解剖或活检组织进行DNA提取。引物指向eb病毒基因组编码衣壳蛋白gp220和Epstein-Barr核抗原1的保守区域。在25例移植患者和所有传染性单核细胞增多症患者的血液中发现特异性EBV扩增。所有出现免疫抑制的淋巴增生性疾病患者（8例器官移植患者和2例获得性免疫缺陷综合征患者）和PCR活检EBV阳性。与免疫抑制无关的19个淋巴瘤或白血病样本中只有1个含有EBV。PCR证实EBV与免疫抑制下发生的淋巴增生性疾病密切相关。在移植患者血液中检测EBV序列的意义，特别是与淋巴细胞增殖的关系，需要进一步研究。
The polymerase chain reaction (PCR) was used to study DNA extracted from the blood of 25 transplant patients, 5 patients with infectious mononucleosis, and 13 healthy subjects and autopsy or biopsy tissue from 29 patients with lymphoproliferative disorders. Primers were directed to conserved regions of the Epstein-Barr virus (EBV) genome encoding capsid protein gp220 and Epstein-Barr nuclear antigen 1. Specific EBV amplification was found in the blood of 11 of 25 transplant patients and all patients with infectious mononucleosis. All patients with lymphoproliferative disorders occurring in the presence of immunosuppression (eight organ transplant patients and two patients with acquired immunodeficieny syndrome) and biopsies positive for EBV by PCR. Only 1 of 19 samples from lymphomas or leukemias unrelated to immunosuppression contained EBV. PCR confirmed the very close association of EBV and lymphoproliferative disorders occurring in the presence of immunosuppression. The significance of detecting EBV sequences in the blood of transplant patients, particularly in relationship to lymphoproliferation, requires further study.