Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules
Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules
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DOI：
10.1095/biolreprod.106.056895
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Ogawa, Takehiko
中科院分区：
文献类型：
--
作者：
Kita, Kaoru;Watanabe, Takeshi;Ogawa, Takehiko
将睾丸生殖细胞移植到生精小管中是目前从给定精原干细胞来源诱导精子发生的唯一方法。在这里，我们展示了一种利用睾丸体细胞自组织能力的替代方法。将胚胎或新生小鼠或大鼠以及新生猪的睾丸细胞解离成单细胞。在植入免疫缺陷小鼠的皮下组织后，它们各自重组成管状结构。当来自精原干细胞并在培养物中扩增的小鼠生殖干（GS）细胞与啮齿类动物的睾丸细胞混合时，它们整合到重建的小管中，并在减数分裂后分化为精子细胞。通过将这些精子细胞显微注射到卵母细胞中产生正常后代。该方法可适用于各种哺乳动物物种，并可用于在异种环境中从 GS 细胞产生功能性配子。
Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells. Here we show an alternative method that harnesses the self-organizing ability of testicular somatic cells. The testicular cells of embryonic or neonatal mice or rats and of newborn pigs were dissociated into single cells. Each of them reorganized into a tubular structure following implantation into the subcutis of immunodeficient mice. When mouse germline stem (GS) cells derived from spermatogonial stem cells and expanded in culture were intermingled with testicular cells of rodents, they were integrated in the reconstituted tubules and differentiated beyond meiosis into spermatids. Normal offspring were produced by the microinjection of those spermatids into oocytes. This method could be applicable to various mammalian species and useful for producing functional gametes from GS cells in a xenoectopic environment.