Short communication: Cellular localization of haptoglobin mRNA in the experimentally infected bovine mammary gland.
Short communication: Cellular localization of haptoglobin mRNA in the experimentally infected bovine mammary gland.
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短通讯：实验感染牛乳腺中触珠蛋白 mRNA 的细胞定位。
DOI：
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发表时间：
2007
影响因子：
3.5
通讯作者：
H. Sauerwein
中科院分区：
文献类型：
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作者：
M. Thielen;M. Mielenz;S. Hiss;H. Zerbe;W. Petzl;H. Schuberth;H. Seyfert;H. Sauerwein
牛乳腺匀浆中证实了触珠蛋白 (Hp) mRNA（牛体内主要急性期蛋白之一）的表达。本研究的目的是在大肠杆菌感染的前 24 小时内，在细胞水平上定位乳房内 Hp mRNA 的表达。为此，随后在屠宰前 6、12 和 24 小时对 3 头牛中的四分之三接种了大肠杆菌；第四节在宰前24小时接受生理盐水作为对照。屠宰后，收集每个季度的组织样本，进行原位杂交和实时逆转录PCR分析。触珠蛋白 mRNA 表达分布于乳腺的肺泡上皮。对组织匀浆中的 Hp mRNA 和牛奶中的 Hp 蛋白进行原位杂交，对 Hp 阳性细胞进行定量，证实感染 24 小时内浓度增加。
Expression of haptoglobin (Hp) mRNA, one of the major acute phase proteins in cattle, was demonstrated in homogenates of the bovine mammary gland. The aim of this study was to localize Hp mRNA expression within the udder at the cellular level during the first 24 h of infection with Escherichia coli. For this purpose, 3 quarters of 3 cows were subsequently inoculated with E. coli at 6, 12, and 24 h preslaughter; the fourth quarter received saline at 24 h preslaughter as a control. After slaughter, tissue samples of each quarter were collected for analyses by in situ hybridization and real-time reverse-transcription PCR. Haptoglobin mRNA expression was allocated to the alveolar epithelium of the mammary gland. Quantification of Hp-positive cells in in situ hybridization of Hp mRNA from tissue homogenates and of Hp protein in milk confirmed increasing concentrations within 24 h of infection.