Hormonal and acid-base regulation of phosphoenolpyruvate carboxykinase mRNA levels in rat kidney.
Hormonal and acid-base regulation of phosphoenolpyruvate carboxykinase mRNA levels in rat kidney.
复制标题
大鼠肾脏中磷酸烯醇丙酮酸羧激酶 mRNA 水平的激素和酸碱调节。
DOI：
10.1016/0003-9861(90)90135-l
复制
发表时间：
1990
影响因子：
3.9
通讯作者：
Mapes,RE
中科院分区：
文献类型：
--
作者：
Watford,M;Mapes,RE
代谢性酸中毒（6天NH 4Cl）导致大鼠肾脏中编码磷酸烯醇式丙酮酸羧激酶的mRNA的相对丰度增加四倍。链脲佐菌素-糖尿病（6天）也导致mRNA丰度增加，但如果通过碳酸氢盐治疗纠正与碳酸氢盐相关的酸中毒，则可以预防这种增加。结果证实，肾磷酸烯醇式丙酮酸羧激酶主要是由酸碱状态的变化，这种控制是在翻译前的步骤。在短期孵育中分离的肾小管已被用于确定哪些药物调节磷酸烯醇式丙酮酸羧激酶mRNA的水平。通过cAMP或cAMP类似物直接作用的糖皮质激素和激素增加了mRNA的相对丰度，但不受通过Ca 2+作用的激素的影响。无论是在pH 7.1的孵育，也没有酸中毒大鼠血清的存在下有任何影响磷酸烯醇式丙酮酸羧激酶mRNA的水平。它的结论是酸中毒，糖皮质激素，cAMP独立调节肾磷酸烯醇式丙酮酸羧激酶的表达。
Metabolic acidosis (6 days NH4Cl) causes a fourfold increase in the relative abundance of mRNA encoding phosphoenolpyruvate carboxykinase in rat kidney. Streptozotocin-diabetes (6 days) also results in an increased abundance of the mRNA but this increase can be prevented if the acidosis associated with bicarbonate is corrected by treatment with bicarbonate. The results confirm that renal phosphoenolpyruvate carboxykinase is regulated primarily by changes in acid-base status and that this control is at a pretranslational step. Isolated kidney tubules in short-term incubation have been used to identify which agents regulate levels of phosphoenolpyruvate carboxykinase mRNA. The relative abundance of the mRNA was increased by glucocorticoids and hormones which act via cAMP, or by cAMP analogues directly, but was not affected by hormones acting via Ca2+. Neither incubation at pH 7.1 nor the presence of serum from acidotic rats had any effect on the level of phosphoenolpyruvate carboxykinase mRNA. It is concluded that acidosis, glucocorticoids, and cAMP independently regulate expression of renal phosphoenolpyruvate carboxykinase.