Effect of selenium supplements on the antioxidant activity and nitrite degradation of lactic acid bacteria
Effect of selenium supplements on the antioxidant activity and nitrite degradation of lactic acid bacteria
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补硒对乳酸菌抗氧化活性及亚硝酸盐降解的影响
DOI：
10.1007/s11274-019-2609-x
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发表时间：
2019-04-01
影响因子：
4.1
通讯作者：
Zhou, Mengzhou
中科院分区：
文献类型：
--
作者：
Chen, Yang;Li, Qin;Zhou, Mengzhou
硒（Se）是人体必需的微量元素之一，富硒乳酸菌（LAB）可以提高无机硒的生物利用价值。本研究的目的是分离富硒乳酸菌，并研究其对抗氧化活性和亚硝酸盐降解的影响。从传统发酵酸菜中分离到耐亚硝酸盐的富硒菌株LAB L.P2，该菌株能在30µg/mL亚硒酸钠（Na2SeO3）培养基中生长。L.P2经16S rDNA鉴定属于植物乳杆菌。当培养基中添加2.0µg/mL na2seo3时，L.P2的生物量和乳酸产量最大，分别为9.52 log CFU/mL和16.99 mg/mL。na2seo3初始浓度为2.0µg/mL时，亚硝酸盐的降解率达到85.76%。富硒LAB增强了L.P2对羟基自由基和超氧自由基的清除能力，提高了脂质过氧化和离子螯合能力。硒4组（添加4.0µg/mL na2seo3）的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性分别达到48.49和50.35 U/mg。因此，Se 4浓度显著高于未添加Se 0组。其中，SOD和GSH-Px酶与亚硝酸盐降解相关（P < 0.01）。综上所述，硒可以增强乳酸菌的抗氧化能力，促进其亚硝酸盐的降解，因此在功能食品中具有潜在的应用前景。
Selenium (Se) is one of the essential trace elements in the human body, and Se-enriched lactic acid bacteria (LAB) can improve the biological utilization value of inorganic Se. The aim of this study was to isolate Se-enriched LAB and study their effects on antioxidant activity and nitrite degradation. The Se-enriched LAB L.P2, which was nitrite-tolerant and could grow in 30 µg/mL sodium selenite (Na2SeO3) medium, was isolated from the traditional fermented Chinese sauerkraut. L.P2 belonged toLactobacillus plantarumaccording to the 16S rDNA analysis. The biomass and lactic acid production of L.P2 reached to a maximum (9.52 log CFU/mL and 16.99 mg/mL) when 2.0 µg/mL Na2SeO3was supplemented in the medium. Additionally, the nitrite degradation rate reached 85.76% when the initial concentration of Na2SeO3was 2.0 µg/mL. The Se-enriched LAB enhanced the scavenging capacity of hydroxyl radical and superoxide free radical of L.P2 and improved the lipid peroxidation and ion-chelating abilities. Moreover, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in Se 4 group (4.0 µg/mL Na2SeO3was added) reached 48.49 and 50.35 U/mg, respectively. Thus, Se 4 concentration was significantly higher than that of Se 0 group (with no Se added). In particular, SOD and GSH-Px enzymes correlated with nitrite degradation (P < 0.01). Collectively, our results indicate that Se supplementation can enhance the antioxidant capacity of LAB, contribute to its nitrite degradation, and thus may have potential applications in functional foods.