FttA is a CPSF73 homologue that terminates transcription in Archaea
FttA is a CPSF73 homologue that terminates transcription in Archaea
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DOI：
10.1038/s41564-020-0667-3
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发表时间：
2020-01
影响因子：
28.3
通讯作者：
Travis J. Sanders;Breanna R. Wenck;Jocelyn N. Selan;Mathew P. Barker;S. A. Trimmer;Julie E. Walker
中科院分区：
文献类型：
--
作者：
Travis J. Sanders;Breanna R. Wenck;Jocelyn N. Selan;Mathew P. Barker;S. A. Trimmer;Julie E. Walker
调控基因表达主要是通过控制必需的多亚基RNA聚合酶转录延伸复合物（TEC）的活性来实现的。TEC转录大基因组区域所需的极端稳定性需要强有力的机制来终止转录。有效的转录终止对于基因密集的细菌和古细菌基因组是特别关键的，其中持续的转录必然会转录紧邻的基因并导致转录和复制装置之间的冲突;转录和翻译的偶联将允许核糖体加载到异常转录物上。只有选择序列或转录终止因子可以破坏否则非常稳定的TEC，我们证明，最后一个普遍保守的古细菌蛋白质与未知的生物功能是终止转录的因子在古细菌（FttA）。FttA解决了原核生物基因结构（操纵子和极性）和真核生物分子同源性（一般转录装置）的二分法，这是观察到的。这种原核和真核转录调控之间的缺失环节为真核生物中参与RNA 3′-末端形成的加工活动的进化提供了最简约的联系。
Regulated gene expression is largely achieved by controlling the activities of essential, multisubunit RNA polymerase transcription elongation complexes (TECs). The extreme stability required of TECs to processively transcribe large genomic regions necessitates robust mechanisms to terminate transcription. Efficient transcription termination is particularly critical for gene-dense bacterial and archaeal genomes, –in which continued transcription would necessarily transcribe immediately adjacent genes and result in conflicts between the transcription and replication apparatuses, –; the coupling of transcription and translation,would permit the loading of ribosomes onto aberrant transcripts. Only select sequences or transcription termination factors can disrupt the otherwise extremely stable TEC and we demonstrate that one of the last universally conserved archaeal proteins with unknown biological function is the Factor that terminates transcription in Archaea (FttA). FttA resolves the dichotomy of a prokaryotic gene structure (operons and polarity) and eukaryotic molecular homology (general transcription apparatus) that is observed in Archaea. This missing link between prokaryotic and eukaryotic transcription regulation provides the most parsimonious link to the evolution of the processing activities involved in RNA 3′-end formation in Eukarya.