Gene expression in gastric biopsies from patients infected with Helicobacter pylori
Gene expression in gastric biopsies from patients infected with Helicobacter pylori
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DOI：
10.1080/00365520410003588
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发表时间：
2004-12-01
影响因子：
1.9
通讯作者：
Correa, H
中科院分区：
文献类型：
--
作者：
Mannick, EE;Schurr, JR;Correa, H
背景：幽门螺杆菌感染对宿主胃粘膜基因表达具有多变的影响，这已通过体外基因芯片分析进行了研究。在这项研究中，检测了幽门螺杆菌感染对患者胃窦粘膜宿主基因表达的影响。方法：对 18 名接受常规内镜评估慢性腹部疾病的未治疗患者进行胃窦活检。 9 名患者有幽门螺杆菌感染的组织学证据，9 名年龄和性别匹配的患者没有幽门螺杆菌感染的组织学证据。使用含有 35,000 个人类表达序列标签的基因芯片对从内窥镜胃窦活检中提取的 RNA 进行微阵列分析，并比较感染和未感染患者的平均基因表达。结果：感染患者粘膜中表达不足的基因包括胃内因子和几种金属硫蛋白亚型。受感染患者粘膜中过度表达的基因包括 MHC II 类分子、免疫球蛋白和 B 细胞激活基因，以及已知诱导细胞凋亡的基因。通过 SYBR green 实时 PCR 证实了一部分基因的表达变化。结论：对幽门螺杆菌感染和未感染患者的胃窦活检进行微阵列分析，揭示了金属调节、免疫和炎症相关基因的差异表达。
Background: Helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. In this study the effects of H. pylori infection on host gene expression in the gastric antral mucosa in patients were examined. Methods: One gastric antral biopsy was obtained from a total of 18 untreated patients undergoing routine endoscopic evaluation of chronic abdominal complaints. Nine patients had histologic evidence of H. pylori infection and 9 age- and sex-matched patients had no histologic evidence of H. pylori infection. A microarray analysis was performed using a gene chip containing 35,000 human expressed sequence tags on RNA extracted from endoscopic, gastric antral biopsies, and average gene expression among infected and uninfected patients was compared. Results: Underexpressed genes in infected patients' mucosa included gastric intrinsic factor and several metallothionein isoforms. Overexpressed genes in infected patients' mucosa comprised MHC Class II molecules, immunoglobulin and B-cell activation genes, as well as genes known to induce apoptosis. Changes in expression were confirmed for a subset of genes by SYBR green real-time PCR. Conclusions: Microarray analysis of antral biopsies from patients with and without H. pylori infection revealed differential expression of metal regulatory, immunity and inflammation-related genes.