The enhancement of cell adherence and inducement of neurite outgrowth of dorsal root ganglia co-cultured with hyaluronic acid hydrogels modified with Nogo-66 receptor antagonist in vitro
The enhancement of cell adherence and inducement of neurite outgrowth of dorsal root ganglia co-cultured with hyaluronic acid hydrogels modified with Nogo-66 receptor antagonist in vitro
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DOI：
10.1016/j.neuroscience.2005.09.029
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Zhao, C
中科院分区：
文献类型：
--
作者：
Hou, S;Tian, W;Zhao, C
制备了多克隆抗nogo -66受体抗体修饰的透明质酸水凝胶，以促进受损中枢神经系统的再生。这些经过修饰的水凝胶不仅可以提供抗体，还可以作为植入受损组织后神经再生的支架。由于未修饰的透明质酸水凝胶不支持细胞附着，因此用多克隆抗nogo -66受体修饰凝胶，目的是改变凝胶的表面性质，从而提高神经元的粘附性和存活率。评价系统的固定化效果后，将鸡背根神经节和背根神经节细胞种植在改性凝胶表面，测定细胞活力。将背根神经节靠近凝胶培养，观察其对神经突生长的诱导作用。在背根神经节和细胞活力实验中，经修饰的水凝胶可使背根神经节和神经元细胞粘附并存活，而未修饰的水凝胶则不能。72h后，神经丝免疫染色阳性。神经突生长诱导试验结果显示，侧背根神经节神经突的数量和长度显著多于侧背根神经节神经突的数量和长度（P < 0.01）。结果表明，抗nogo -66受体修饰的透明质酸水凝胶可以支持神经细胞的体外附着和存活。此外，该系统可以极大地诱导神经突起的生长。结果还表明，该修饰水凝胶具有修复中枢神经系统损伤的潜力。(c) 2005 IBRO。Elsevier Ltd.出版。版权所有。
Hyaluronic acid hydrogels modified with polyclonal anti-Nogo-66 receptor antibody were developed in order to promote regeneration in the injured CNS. These modified hydrogels were intended not only to deliver antibodies, but also to serve as a scaffold for neural regeneration following their implantation into injured tissue. Since unmodified hyaluronic acid-hydrogels do not support cell attachment, the gels were modified with polyclonal anti-Nogo-66 receptor with the aim of altering the surface properties of the gels in such a way as to improve neuronal adherence and survival. After evaluating the immobilization efficiency of the system, chicken dorsal root ganglia and dorsal root ganglia cells were planted on the surface of the modified gels to determine cell viability. Dorsal root ganglia were also cultured close to the gels in order to assay the inducement of neurite outgrowth. In dorsal root ganglia and cell viability assay, dorsal root ganglia and neuron cells could adhere to the modified hydrogels and survive well, but it did not happen to unmodified hydrogels. After 72 h, these attached cells were stained positively with immuno-staining for neurofilament. Neurite outgrowth inducement assay showed that the number and length of dorsal root ganglia neurites on the side toward modified hydrogels were significantly more than that on the opposite side (both P < 0.01). The results reveal that hyaluronic acid-hydrogels modified with anti-Nogo-66 receptor can support neural cell attachment and survival in vitro. Furthermore, this system can greatly induce neurite outgrowth. The results also indicate that this modified hydrogels have potential to repair injury in the CNS. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.