Interprotein metal exchange reactions of metallothionein.
Interprotein metal exchange reactions of metallothionein.
复制标题
金属硫蛋白的蛋白质间金属交换反应。
DOI：
10.1007/978-3-0348-6784-9_10
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Hilt,CR
中科院分区：
文献类型：
--
作者：
Otvos,JD;Engeseth,HR;Nettesheim,DG;Hilt,CR
在体外制备兔肝~(113)Cd、~(113)Cd、~(111)Cd、~(113)Cd、~(11)Cd对这一迄今未知的反应的动力学研究表明，即使在低生理浓度下，如果不快速交换金属以产生混合金属蛋白质物种，镉和锌就不可能共存，其中镉和锌分别优先位于4-金属和3-金属簇中。还研究了Cd在7个结合位之间的自交换反应，发现3金属簇内Cd交换非常快(时间尺度为秒)，而4金属簇间或簇间Cd交换要慢几个数量级。
Different methods of preparing rabbit liver113Cd,ZnMTin vitrowere investigated in an attempt to reproduce the distinctive and reproducible manner in which the metals are distributed between the 2 clustersin vivoin protein produced in response to Cd administration.113Cd NMR was used to demonstrate that the native metal distribution is not produced by Cd displacement of Zn from ZnMT, but rather by a direct metal interchange reaction between CdMT and ZnMT. Kinetic studies of this heretofore unknown reaction indicate that even at low physiological concentrations, CdMT and ZnMT cannot coexist without rapidly exchanging metals to produce mixed-metal protein species containing Cd and Zn located preferentially in the 4- and 3-metal clusters, respectively. The self-exchange reaction of Cd among the 7 binding sites of CdMT was also investigated and it was found that exchange of Cd among sites in the 3-metal cluster was very rapid (time-scale of seconds) while among sites of the 4-metal cluster or between clusters it was several orders of magnitude slower.