SODIUM-INDUCED CALCIUM RELEASE FROM MITOCHONDRIA IN BROWN ADIPOSE-TISSUE
SODIUM-INDUCED CALCIUM RELEASE FROM MITOCHONDRIA IN BROWN ADIPOSE-TISSUE
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DOI：
10.1073/pnas.76.5.2350
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
CANNON, B
中科院分区：
文献类型：
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作者：
ALSHAIKHALY, MHM;NEDERGAARD, J;CANNON, B
如果有底物存在，[仓鼠]棕色脂肪组织的偶联线粒体可以积累钙离子。钙离子通过加入20 mM的Na+而不是通过添加K+或胆碱+来释放。能量耗散钠离子引起的钙循环最大值为20 mM和10微米。在棕色脂肪细胞中，如果添加底物，钙离子载体A23187和钠离子载体Monensin会增加呼吸，而在低Na+缓冲液中孵育会降低去甲肾上腺素诱导的呼吸。Na+诱导的Ca~(2+)释放发生在棕色脂肪组织中；释放的Ca~(2+)可能具有调节或发热作用，或两者兼而有之。
Coupled mitochondria of [hamster] brown adipose tissue can accumulate Ca2+ if a substrate is present. The Ca2+ is released by addition of 20 mM Na+, but not by addition of K+ or choline+. Energy-dissipating Na+ induced Ca2+ cycling occurs maximally with 20 mM Na+ and 10 .mu.M Ca2+. In brown adipocytes, the Ca2+ionophore A23187 and the Na+ ionophore monensin increase respiration if substrate is added and incubation in a low-Na+ buffer decreases norepinephrine induced respiration. The Na+ induced Ca2+ release takes place in brown adipose tissue; released Ca2+ could have a regulatory or thermogenic role or both.