Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
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DOI：
10.1364/ao.38.006994
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发表时间：
1999-12-01
期刊：
影响因子：
1.9
通讯作者：
Depeursinge, C
中科院分区：
文献类型：
--
作者：
Cuche, E;Marquet, P;Depeursinge, C
提出了一种用CCD摄像机作为记录装置的全息显微术的数字化方法。利用菲涅耳近似下标量衍射的计算方法，从振幅和相位两个方面对显微物体的放大像记录的离轴全息图进行了数值再现。对于相衬成像，重建方法涉及参考波的数字副本的计算。本文提出了一种校正相位像差的数字方法。我们提出了一个详细的描述的重建过程，并表明，横向分辨率等于衍射极限的成像系统。(C)1999年美国光学学会OCIS代码：090.0090、110.0180、040.1520、350.5030、090.1000。
We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scaler diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system. (C) 1999 Optical Society of America OCIS codes: 090.0090, 110.0180, 040.1520, 350.5030, 090.1000.