Understanding SERS of bacteria
Understanding SERS of bacteria
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DOI：
10.1002/jrs.2121
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发表时间：
2009-03-01
影响因子：
2.5
通讯作者：
Zeiri, L.
中科院分区：
文献类型：
--
作者：
Efrima, S.;Zeiri, L.
表面增强拉曼光谱（Sers）由于其高的指纹（振动）信息含量、极高的灵敏度（低至单分子水平）和对生物系统固有的水环境的遗忘性，已被认为是鉴定细菌的有力工具。在这里，我们回顾了各种研究，试图利用Sers这一重要目标，根据我们自己的小组在过去10年左右的工作进行了比较。我们表明，Sers有一个额外的主要优势，即，它引入了一个新的维度的选择性，这一方面，使它更适合作为一种分析工具，但另一方面，它需要获得控制的精确方式，其中SERS活性金属中心的生产和接触的微生物。我们在这篇评论中的重点是理解的SERS活性中心的性质和它们在细菌内的位置方面的光谱。在光谱的解释和分配时，我们始终牢记细菌鉴定的最终目标。版权所有（C）2008约翰威利父子有限公司
Surface-enhanced Raman spectroscopy (SERS) has been suggested as a powerful tool to identify bacteria, drawing from its high fingerprint (vibrational) information content, its extreme sensitivity (down to the single molecule level) and its obliviousness to the aqueous environment intrinsic to biological systems. We review here in a comparative manner the various studies that attempted to utilize SERS for this important goal in light of the work carried out by our own group over the past 10 years or so. We show that SERS has an additional major advantage, namely, it introduces a new dimension of selectivity, which, on the one hand, makes it even more suitable as an analytical tool, but on the other hand, it requires gaining control of the precise manner in which the SERS-active metal centers are produced and brought into contact with the micro-organism. Our emphasis in this review is on understanding the spectra in terms of the nature of the SERS-active centers and their placement within the bacterium. On the interpretation and assignment of the spectra, we constantly keep in mind the final goal of bacteria identification. Copyright (C) 2008 John Wiley & Sons, Ltd.