Trigonelline hydrochloride: A promising inhibitor for type I collagen fibrillation
Trigonelline hydrochloride: A promising inhibitor for type I collagen fibrillation
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DOI：
10.1016/j.colsurfb.2018.06.030
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发表时间：
2018-10-01
影响因子：
5.8
通讯作者：
Fathima, N. Nishad
中科院分区：
文献类型：
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作者：
Rasheeda, K.;Fathima, N. Nishad
胶原蛋白是一种主要的结构蛋白，具有低毒性、低免疫反应和促进细胞生长等特性。如果胶原蛋白积累过多，尤其是在心肌中，则会导致纤维化，进而损害心肌组织的正常功能。为了克服胶原纤维颤动，本研究中使用盐酸葫芦巴碱作为抑制 I 型胶原自发自组装的潜在药物。胡芦巴碱盐酸盐是一种烟酸衍生物，主要存在于胡芦巴中。浊度测定的实验工作表明，与天然胶原蛋白相比，滞后期有所增加，表明纤维颤动的延迟。根据流变学方面，胶原复合物的粘度由于剪切速率的增加而降低，从而解开聚集的胶原原纤维颗粒并使其沿着速率方向排列。通过 AFM 和 HR-SEM 进行的形态学评估表明，随着盐酸葫芦巴碱浓度的增加，纤维颤动可有效减少。 FT-IR (ATR) 研究表明，用盐酸葫芦巴碱处理后，二级结构螺旋性变得紧密。葫芦巴碱对胶原纤维生成的这种有希望的作用可以通过创新的药物输送系统明智地用于靶向胶原蛋白积累的特定位点。
Collagen is a major structural protein, with properties such as low toxicity, low immune response and promoting cellular growth. If there is an excess of collagen accumulation, especially in the myocardium it leads to fibrosis, which in turn impairs the normal functioning of the myocardial tissues. In order to overcome collagen fibrillation, trigonelline hydrochloride has been used in this study as a potential agent for inhibiting the spontaneous self-assembly of type I collagen. Trigonelline hydrochloride, a nicotinic acid derivative is largely found in Trigonella foenum-graectrin L. (fenugreek). Experimental work on turbidity assay shows that there is an increase in the lag phase compared to the native collagen indicating a delay in fibrillation. According to theological aspects, viscosity of the collagen composite decreases due to the increase in shear rate, which disentangles the aggregated particles of collagen fibrils and allows it to align along the direction of rate. Morphological assessments through AFM and HR-SEM suggest that there is an effective reduction in fibrillation with an increase in the concentration of trigonelline hydrochloride. FT-IR (ATR) studies indicate that compactness of secondary structure helicity occurs on treatment with trigonelline hydrochloride. Such promising effect of trigonelline on collagen fibrillogenesis can be judiciously used for targeting specific sites of collagen accumulation via innovative drug delivery systems.