Flexible electrical recording from cells using nanowire transistor arrays
Flexible electrical recording from cells using nanowire transistor arrays
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DOI：
10.1073/pnas.0902752106
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发表时间：
2009-05-05
影响因子：
11.1
通讯作者：
Lieber, Charles M.
中科院分区：
文献类型：
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作者：
Cohen-Karni, Tzahi;Timko, Brian P.;Lieber, Charles M.
半导体纳米线（NWs）具有独特的电子特性和可与参与细胞通信的生物结构相媲美的尺寸，因此使其成为与生物系统建立活性界面的有前途的纳米结构。我们报道了一种将NW场效应晶体管（NWFET）与细胞结合的灵活方法，并证明了硅NWFET阵列与胚胎鸡心肌细胞的耦合。将心肌细胞培养在透明的聚二甲基硅氧烷（PDMS）薄片上，然后与在标准衬底上制备的Si-NWFET阵列接触。从心肌细胞中记录的NWFET电导信号表现出优异的信噪比，其值通常为bbb50，信号幅度可以通过改变水门电压电位V-g来改变器件灵敏度。在-0.5至-0.1 V的V-g范围内，从心肌细胞记录的信号显示出31至7 nS的振幅变化，而校准电压保持不变，表明NWFET/细胞界面稳健。此外，作为PDMS/电池支架在器件芯片上位移增加/减少的函数记录的信号显示，信号幅度（校准电压）从31 nS （1.0 mV）增加到72 nS (2.3 mV)，可逆地增加了100倍。在接近但低于细胞破裂点的位移研究中，校准后的信号振幅可达10.5 +/- 0.2 mV。最后，对连接到心肌细胞单层的NWFET阵列的信号进行多路记录，可以以良好的空间和时间分辨率确定时间位移和信号传播。我们的模块化方法简化了将心肌细胞和其他细胞连接到高性能si -NWFET的过程，从而增加了NWFET阵列的实验多功能性，并实现了亚细胞水平的器件注册。
Semiconductor nanowires (NWs) have unique electronic properties and sizes comparable with biological structures involved in cellular communication, thus making them promising nanostructures for establishing active interfaces with biological systems. We report a flexible approach to interface NW field-effect transistors (NWFETs) with cells and demonstrate this for silicon NWFET arrays coupled to embryonic chicken cardiomyocytes. Cardiomyocyte cells were cultured on thin, optically transparent polydimethylsiloxane (PDMS) sheets and then brought into contact with Si-NWFET arrays fabricated on standard substrates. NWFET conductance signals recorded from cardiomyocytes exhibited excellent signal-to-noise ratios with values routinely >5 and signal amplitudes that were tuned by varying device sensitivity through changes in water gate-voltage potential, V-g. Signals recorded from cardiomyocytes for V-g from -0.5 to -0.1 V exhibited amplitude variations from 31 to 7 nS whereas the calibrated voltage remained constant, indicating a robust NWFET/cell interface. In addition, signals recorded as a function of increasing/decreasing displacement of the PDMS/cell support to the device chip showed a reversible >2x increase in signal amplitude (calibrated voltage) from 31 nS (1.0 mV) to 72 nS (2.3 mV). Studies with the displacement close to but below the point of cell disruption yielded calibrated signal amplitudes as large as 10.5 +/- 0.2 mV. Last, multiplexed recording of signals from NWFET arrays interfaced to cardiomyocyte monolayers enabled temporal shifts and signal propagation to be determined with good spatial and temporal resolution. Our modular approach simplifies the process of interfacing cardiomyocytes and other cells to high-performance Si-NWFETs, thus increasing the experimental versatility of NWFET arrays and enabling device registration at the subcellular level.