RAPDs and allozymes exhibit similar levels of diversity and differentiation among populations and races of Douglas-fir
RAPDs and allozymes exhibit similar levels of diversity and differentiation among populations and races of Douglas-fir
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DOI：
10.1046/j.1365-2540.1998.00355.x
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发表时间：
1998-07-01
期刊：
影响因子：
3.8
通讯作者：
Strauss, SH
中科院分区：
文献类型：
--
作者：
Aagaard, JE;Krutovskii, KV;Strauss, SH
利用36个核基因编码的RAPD位点和20个等位酶位点，对分布广泛的北美针叶树花旗松（Pseudotsuga menziesii（Mirb.）Franco]。RAPD分析使用二倍体种子胚DNA从22至36棵树在每个6个人口抽样的三个主要种族（两个种族的人口）。一个可比的等位酶数据集附近的人群构建了一个已发表的研究。RAPD的细胞器起源被排除在与叶绿体和线粒体DNA富集探针杂交的印迹RAPD凝胶。RAPD和等位酶标记在居群内的多样性水平（H-S = 0.22 ± 0.03和0.16 ± 0.03）和居群间的分化水平（G（ST）= 0.34 ± 0.07和0.29 ± 0.07）相近。当等位酶数据集转化为显性，双等位基因标记，以研究如何RAPDs可能偏见的多样性估计，restaurant研究表明，模拟的H-S和H-T减少了一半，无论样本量。因为观察到的多样性RAPDs相当于，或高于，等位酶，我们的模拟表明，RAPD标记可能包含更高水平的固有的，但隐藏的多样性。与此相反，模拟表明，使用RAPD的G（ST）的估计值在我们采用的人口规模上不应有显着偏差。
Thirty-six nuclear-encoded RAPD loci and 20 allozyme loci were studied to compare levels of diversity and differentiation among populations and races of the widespread North American conifer, Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. RAPD assays used diploid seed embryo DNA from 22 to 36 trees in each of six populations that sampled the three major races (two populations per race). A comparable allozyme data set for nearby populations was constructed from a published study. RAPDs of organelle origin were excluded by hybridization of blotted RAPD gels with chloroplast and mitochondrial DNA-enriched probes. RAPD and allozyme markers had similar levels of diversity within populations (H-S = 0.22+/-0.03 and 0.16+/-0.03, respectively) and differentiation among populations (G(ST) = 0.34+/-0.07 and 0.29+/-0.07, respectively). When the allozyme data set was transformed into dominant, biallelic markers to study how RAPDs may bias diversity estimates, resampling studies showed that simulated H-S and H-T were reduced by half regardless of sample size. Because observed diversity for RAPDs was equivalent to, or higher than, that of allozymes, our simulations suggest that RAPD markers may contain substantially higher levels of inherent, but hidden, diversity. In contrast, the simulations showed that estimates of G(ST) using RAPDs should not be significantly biased at the population sizes we employed.