Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Coagulase-Negative Staphylococcus/Mammaliicoccus from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene fosB.
Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Coagulase-Negative Staphylococcus/Mammaliicoccus from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene fosB.
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DOI：
10.3390/pathogens11040469
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发表时间：
2022-04-14
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葡萄球菌是污染零售肉制品的主要细菌种类。本研究的目的是阐明日本零售碎肉中葡萄球菌/哺乳动物球菌属的流行率、耐药性和遗传决定因素。在五个月内购买的146个零售绞肉样本（鸡肉、猪肉、混合牛肉/猪肉）中，金黄色葡萄球菌和112株凝固酶阴性球菌（CoNS）/哺乳动物球菌，包括20个物种。S.金黄色葡萄球菌分离株分为五种遗传类型，即，coa-IIa/ST 5、coa-VIc/ST 352（CC 97）、coa-VIIb/ST 398、coa-Xa/ST 15和coa-XIc/ST 9，与家畜相关克隆的相关性较好。所有葡萄球菌分离株均为mecA阴性，除氨苄西林外，其余对所有抗菌药物均敏感。金黄色葡萄球菌（耐药率50%）。在CoNS中，磷霉素抗性基因fosB是普遍存在的（30/112; 26.8%），主要在S. capitis、S. warneri和S.萨皮提库斯fosB的系统发育分析显示，存在7个簇，显示出广泛的多样性与65-81%的同一性之间的不同簇。在来自碎肉样品的CoNS分离株中，fosB被分为三个簇，S. sachticus的fosB具有3个遗传群，差异最大。这些发现表明，多个携带fosB的质粒在一些CoNS物种之间的循环。
Staphylococcus is a major bacterial species that contaminates retail meat products. The objective of this study was to clarify the prevalence, antimicrobial resistance and genetic determinants of Staphylococcus/Mammaliicoccus species in retail ground meat in Japan. From a total of 146 retail ground meat samples (chicken, pork, mixed beef/pork) purchased during a 5-month period, 10 S. aureus and 112 isolates of coagulase-negative staphylococcus (CoNS)/Mammaliicoccus comprising 20 species were recovered. S. aureus isolates were classified into five genetic types, i.e., coa-IIa/ST5, coa-VIc/ST352 (CC97), coa-VIIb/ST398, coa-Xa/ST15, and coa-XIc/ST9, which were all related to those of livestock-associated clones. All the staphylococcal isolates were mecA-negative and mostly susceptible to all the antimicrobials tested, except for ampicillin among S. aureus (resistance proportion; 50%). Among CoNS, the fosfomycin resistance gene fosB was prevalent (30/112; 26.8%), primarily in S. capitis, S. warneri, and S. saprophyticus. Phylogenetic analysis of fosB revealed the presence of seven clusters, showing broad diversity with 65–81% identity among different clusters. In the CoNS isolates from ground meat samples, fosB was assigned into three clusters, and S. saprophyticus harbored the most divergent fosB with three genetic groups. These findings suggested the circulation of multiple fosB-carrying plasmids among some CoNS species.