Specific Volatile Compounds from Mango Elicit Oviposition in Gravid Bactrocera dorsalis Females
Specific Volatile Compounds from Mango Elicit Oviposition in Gravid Bactrocera dorsalis Females
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DOI：
10.1007/s10886-014-0403-7
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发表时间：
2014-03-01
影响因子：
2.3
通讯作者：
Bruce, Toby J. A.
中科院分区：
文献类型：
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作者：
Jayanthi, Pagadala D. Kamala;Kempraj, Vivek;Bruce, Toby J. A.
选择一个合适的产卵地点对雌性昆虫的健康至关重要，因为它决定了它们后代的成功发育。在产卵过程中，昆虫必须利用外部环境的线索来做出产卵地点的适当选择。通才型昆虫可以探测到大量的线索并作出反应，但在选择压力下，它们会采用最可靠的线索来压倒噪音，提高寻找宿主的效率。东方果蝇小实蝇（Bactrocera dorsalis, Hendel）是一种多面手，利用多种水果作为产卵地点。然而，对桔色小蠊产卵刺激剂的特性和性质尚不清楚。最近，我们发现了一种挥发性化合物。-八氯内酯，在背蝇中引起先天的产卵反应。我们从芒果中筛选了21种ead活性挥发物，以检测它们的促排卵活性。1-辛烯-3-醇、甲酰乙酯和苯并噻唑能诱导背背小蠊妊娠雌虫产卵。果蝇将大部分卵放入含有促排卵剂的纸浆盘中，只有一小部分卵被放入对照盘中。在二元选择产卵试验中，95.1、93.7和65.6%的卵产在1-辛烯-3-醇、甲酰乙酯和苯并噻唑处理过的盘上。单片双选择实验证明，促排卵剂在雌性背背小蠊妊娠后的产卵地点选择中起重要作用。在模拟的半自然实验中，怀孕的背背圆蚧雌性准确地区分了含有和不含有1-辛烯-3-醇、甲酰乙酯和。通过在处理过的水果上产下更多的卵。然而，在这种情况下，苯并噻唑并没有引起产卵的增加。我们的研究结果表明，鉴定的促排卵剂是“关键”化合物，果蝇将其与合适的产卵地点联系在一起。
Selecting a suitable oviposition site is crucial to the fitness of female insects because it determines the successful development of their offspring. During the oviposition process, an insect must use cues from the external environment to make an appropriate choice of where to lay eggs. Generalist insects can detect and react to a plethora of cues, but are under selection pressure to adopt the most reliable ones to override noise and increase efficiency in finding hosts. The oriental fruit fly, Bactrocera dorsalis (Hendel), is a generalist that utilizes a multitude of fruits as oviposition sites. However, the identity and nature of oviposition stimulants for B. dorsalis is not well known. Recently, we identified a volatile compound.-octalactone that elicits an innate oviposition response in B. dorsalis. We screened 21 EAD-active volatiles, identified from mango, for their oviposition stimulant activity. 1-Octen-3-ol, ethyl tiglate, and benzothiazole instigated oviposition in gravid B. dorsalis females. Flies deposited most of their eggs into pulp discs with oviposition-stimulants, and only a small fraction of eggs were laid into control discs. In a binary choice oviposition assay, 95.1, 93.7, and 65.6 % of eggs were laid in discs treated with 1-octen-3-ol, ethyl tiglate, and benzothiazole, respectively. Single plate two-choice assays proved that oviposition-stimulants were crucial in oviposition site selection by gravid female B. dorsalis. In simulated semi-natural assays, gravid B. dorsalis females accurately differentiated between fruits with and without 1-octen-3-ol, ethyl tiglate, and.-octalactone by laying more eggs on the treated fruit. However, benzothiazole did not elicit an increase in oviposition when presented in this context. Our results suggest that the identified oviposition-stimulants are 'key' compounds, which the flies associate with suitable oviposition sites.