FORMALDEHYDE AS A PRETREATMENT FOR DERMAL COLLAGEN HETEROGRAFTS
FORMALDEHYDE AS A PRETREATMENT FOR DERMAL COLLAGEN HETEROGRAFTS
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DOI：
10.1016/0304-4165(80)90335-9
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
STEPHEN, L
中科院分区：
文献类型：
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作者：
BARKER, H;OLIVER, R;STEPHEN, L
以~（14）C-甲醛为示踪剂，研究了经胰蛋白酶纯化的猪真皮胶原蛋白与不同浓度甲醛交联后的制备、体内外稳定性及对细菌胶原酶的抗性。在37 ℃磷酸盐缓冲盐水中洗涤。在达到稳定性之前，C产生超过6周的甲醛快速损失。洗涤19周后，剩余12-20%的初始放射性，分别代表与0.1、1和5%甲醛反应21天后6、18和35 μ mol甲醛/g胶原。胶原蛋白，掺入稳定结合的甲醛产生的反应与甲醛的浓度为0.5%或以上，是完全抵抗细菌胶原酶。甲醛交联的稳定作用也通过在大鼠中植入纤维状猪真皮胶原蛋白来证明。8周后，在所有植入物中保留了大量的甲醛。在24周的时间内，用1%甲醛预处理时没有净质量损失，但用0.1%甲醛预处理时有一些损失。
The preparation, stability both in vitro and in vivo and resistance to bacterial collagenase of trypsin-purified pig dermal collagen, cross-linked with a range of concentrations of formaldehyde in phosphate-buffered saline, was studied using 14C-labeled formaldehyde as a tracer. Washing in phosphate-buffered saline at 37.degree. C produced rapid loss of formaldehyde over 6 wk before stability was reached. After 19 wk washing 12-20% of the initial radioactivity remained, representing 6, 18 and 35 .mu.mol formaldehyde/g of collagen after 21 days reaction with 0.1, 1 and 5% formaldehyde, respectively. Collagen, incorporating stable-bound formaldehyde arising from reaction with formaldehyde in concentrations of 0.5% or over, was totally resistant to bacterial collagenase. The stabilizing effect of formaldehyde cross-linking was also demonstrated by implants of fibrous pig dermal collagen in rats. After 8 wk a significant constant amount of formaldehyde was retained in all implants. There was no net loss of mass over a 24 wk period when pre-treated with 1% formaldehyde, but some loss when pre-treated with 0.1% formaldehyde.