Proteomic Changes in Newly Synthesized Brassica napus Allotetraploids and Their Early Generations
Proteomic Changes in Newly Synthesized Brassica napus Allotetraploids and Their Early Generations
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新合成甘蓝型油菜异源四倍体及其早期代的蛋白质组变化
DOI：
10.1007/s11105-011-0301-x
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发表时间：
2011-12-01
影响因子：
2.1
通讯作者：
Wang, Youping
中科院分区：
文献类型：
--
作者：
Kong, Fang;Mao, Shanjing;Wang, Youping
多倍体是高等植物中的一个重要过程，通常被描述为可能诱导胁迫和防御反应的基因组休克。以甘蓝型油菜异源四倍体为模型，研究异源多倍体形成过程中蛋白质组的变化。B叶片蛋白质组的大规模分析。与纯合二倍体祖细胞芜菁和甘蓝进行比较，并在B中进行蛋白质组变化。早代（F1-F4）。F1代中所有这些差异表达蛋白的丰度与其祖细胞中表达的相应蛋白不同，其中一些相对偏离中亲预测，表现出一定程度的非加性表达再模式。 虽然叶蛋白在合成B中被广泛修饰。在油菜中，“管家”蛋白的分布没有受到干扰。此外，在两个基因组合并后，没有观察到混乱或大混乱的证据。相反，一种新的秩序迅速发展起来，可能会在合成B的后代中进化。油菜。
Polyploidy is a prominent process in higher plants and is often described as a genomic shock that may induce stress and defense responses. The Brassica napus allotetraploid model was chosen to investigate the proteomic modifications that occur during allopolyploid formation. Large-scale analysis of the proteome from the leaves of B. napus was performed and compared with the homozygous diploid progenitors, Brassica rapa and Brassica oleracea, and among the proteomic changes in B. napus in the early generations (F1–F4). The abundance of all these differentially expressed proteins in the F1 generation differed from that of the corresponding proteins expressed in its progenitors, some of which relatively deviated from mid-parent predictions, exhibiting somewhat non-additive expression repatterning. Although leaf proteins were extensively modified in synthetic B. napus, the distribution of the “housekeeping” proteins was not disturbed. Moreover, no evidence of chaos or large disorder was observed after the merging of the two genomes. Instead, a novel order quickly developed, which might evolve in further generations of synthetic B. napus.