THE POLIOVIRUS RECEPTOR PROTEIN IS PRODUCED BOTH AS MEMBRANE-BOUND AND SECRETED FORMS
THE POLIOVIRUS RECEPTOR PROTEIN IS PRODUCED BOTH AS MEMBRANE-BOUND AND SECRETED FORMS
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DOI：
10.1002/j.1460-2075.1990.tb07520.x
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发表时间：
1990-10-01
期刊：
影响因子：
11.4
通讯作者：
NOMOTO, A
中科院分区：
文献类型：
--
作者：
KOIKE, S;HORIE, H;NOMOTO, A
分别从HeLa S3细胞基因组文库和互补DNA文库中分离到编码脊髓灰质炎病毒受体的基因组和互补DNA克隆。对这些克隆的DNA的核苷酸序列分析表明，脊髓灰质炎病毒受体基因是.apprx。20kb长，在编码区包含七个内含子，至少四个涉及编码序列的mRNA异构体通过交替剪接产生，并似乎编码四个不同的分子，即PVR.α、PVR.β、PVR.Gamma。PVR三角洲..预测的氨基酸序列表明PVR.Alpha。和PVR，分别对应于前面描述的cDNA克隆H20A和H20B，是完整的膜蛋白，而这里描述的另外两个分子首次缺乏假定的跨膜结构域。携带PVR.Alpha的小鼠细胞转化子。对于脊髓灰质炎病毒感染是允许的，但那些携带PVR.beta的人。几乎是不允许的。与PVR.Alpha不同，PVR.beta。在小鼠细胞转化子表面没有检测到，但通过使用脊髓灰质炎病毒受体的单抗的免疫学方法在培养液中检测到。PVR.Alpha、PVR.Beta三种剪接产品。和PVR.Gamma。在人脑、白细胞、肝脏、肺和胎盘的Poly(A)+RNA中使用适当的引物进行聚合酶链式反应检测；所使用的引物的选择不允许检测PVR。以基因片段为探针的原位杂交结果表明，PVR基因位于q13.1fwdarw带。人类19号染色体的q13.2。
Both genomic and complementary DNA clones encoding poliovirus receptors were isolated from genomic and complementary DNA libraries prepared from HeLa S3 cells, respectively. Nucleotide sequence analysis of these cloned DNAs revealed that the poliovirus receptor gene is .apprx. 20 kb long and contains seven introns in the coding region, and that at least four mRNA isoforms referring to the coding sequence are generated by alternative splicing and appear to encode four different molecules, that is, PVR.alpha., PVR.beta., PVR.gamma. PVR.delta.. The predicted amino acid sequences indicate that PVR.alpha. and PVR.delta., corresponding to the previously described cDNA clones H20A and H20B, respectively, are integral membrane proteins while the other two molecules described here for the first time lack a putative transmembrane domain. Mouse cell transformants carrying PVR.alpha. were permissive for poliovirus infection, but those carrying PVR.beta. were hardly permissive. In contrast to PVR.alpha., PVR.beta. was not detected on the surface of the mouse cell transformants but was detected in the culture fluid by an immunological method using a monoclonal antibody against poliovirus receptor. Three types of splicing products for PVR.alpha., PVR.beta. and PVR.gamma. were detected by polymerase chain reactions using appropriate primers in poly(A)+ RNAs of the brain, leukocyte, liver, lung and placenta of humans; the choice of primers used did not permit detection of PVR.delta.. In situ hybridization using a cDNA fragment as a probe demonstrated that the PVR gene is located at the band q13.1 .fwdarw. q13.2 of human chromosome 19.