Biodegradation of 2,4,6-trinitrophenol by Rhodococcus sp. isolated from a picric acid-contaminated soil.
Biodegradation of 2,4,6-trinitrophenol by Rhodococcus sp. isolated from a picric acid-contaminated soil.
复制标题
DOI：
10.1016/j.jhazmat.2008.07.086
复制
发表时间：
2009-04
影响因子：
13.6
通讯作者：
Jinyou Shen;Jianfa Zhang;Y. Zuo;Lianjun Wang;Xiuyun Sun;Jiansheng Li;Weiqing Han;R. He
中科院分区：
文献类型：
--
作者：
Jinyou Shen;Jianfa Zhang;Y. Zuo;Lianjun Wang;Xiuyun Sun;Jiansheng Li;Weiqing Han;R. He
从苦味酸污染土壤中分离到一株苦味酸降解菌NJUST 16，经16 SrRNA序列分析鉴定为红球菌属（Rhodococcus sp.）。降解试验表明，菌株NJUST 16能够利用苦味酸作为唯一的碳源、氮源和能量源。该分离物在30°C和初始pH 7.0-7.5下在补充有苦味酸的矿物盐培养基中生长最佳。这与该菌株对苦味酸的降解基本一致。酵母膏和蛋白胨等氮源的加入加速了苦味酸的降解。然而，刺激是浓度依赖性的。降解伴随着化学计量的亚硝酸盐和酸化的释放。苦味酸在相对高浓度（>3.93mM）下的降解表明，降解是pH和亚硝酸盐依赖的。中性和微碱性pH对于NJUST 16菌株实现高浓度苦味酸降解至关重要。
A picric acid-degrading bacterium, strain NJUST16, was isolated from a soil contaminated by picric acid and identified as a member of Rhodococcus sp. based on 16S rRNA sequence. The degradation assays suggested that the strain NJUST16 could utilize picric acid as the sole source of carbon, nitrogen and energy. The isolate grew optimally at 30°C and initial pH 7.0–7.5 in the mineral salts medium supplemented with picric acid. It was basically consistent with degradation of picric acid by the isolate. Addition of nitrogen sources such as yeast extract and peptone accelerated the degradation of picric acid. However, the stimulation was concentration dependent. The degradation was accompanied by release of stoichiometric amount of nitrite and acidification. The degradation of picric acid at relatively high concentrations (>3.93mM) demonstrated that the degradation was both pH and nitrite dependent. Neutral and slightly basic pH was crucial to achieve high concentrations of picric acid degradation by the NJUST16 strain.