Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp and Stenotrophomonas sp cells
Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp and Stenotrophomonas sp cells
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DOI：
10.1016/j.jenvman.2015.04.011
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发表时间：
2015-07-01
影响因子：
8.7
通讯作者：
Dong, Xinjiao
中科院分区：
文献类型：
--
作者：
Ge, Shimei;Ge, Shichao;Dong, Xinjiao
生物修复是一种有效的去除废水中毒性很强的六价铬（Cr（VI））的方法。从Cr（VI）污染土壤中分离到2株能有效还原Cr（VI）的细菌，经16 S rRNA基因序列分析，分别鉴定为短杆菌属（Brevibacterium sp. K1）和寡养单胞菌属（Stenotrophomonas sp. D 6）。短杆菌K1和寡养单胞菌D 6分别能在含1000和1600 mg/L K2 Cr2 O 7的Luria肉汤培养基中生长，在含200 mg/L K2 Cr2 O 7的LB培养基中72 h内完全还原Cr（VI）。进一步的分析表明，透化K1和D 6细胞减少Cr（VI）更有效地比休息细胞。Triton X-100是所测试的最佳透化剂。两种菌株的透化细胞在需要补充之前可以两次完全还原工业废水中的Cr（VI）。结果表明，这些铬酸盐还原菌是潜在的候选人，为实际使用的生物处理含Cr（VI）的工业废水与寡养单胞菌属D 6是更有效的细菌。(C)2015爱思唯尔有限公司版权所有。
Bioremediation has been found to be a useful method for removing hexavalent chromium (Cr(VI)), which is very toxic, from wastewater. Two strains of bacteria that were able to reduce Cr(VI) effectively were isolated from Cr(VI) contaminated soil samples and identified as Brevibacterium sp. K1 and Stenotrophomonas sp. D6, respectively, based on 16S rRNA gene sequence analyses. Brevibacterium sp. K1 and Stenotrophomonas sp. D6 could grow in Luria Broth medium containing K2Cr2O7 at 1000 and 1600 mg/L, respectively, and they completely reduced the Cr(VI) in LB medium containing K2Cr2O7 at 200 mg/L within 72 h. Further analyses revealed that permeabilized K1 and D6 cells reduced Cr(VI) more effectively than did the resting cells. Triton X-100 was the best permeabilizing agent that was tested. The permeabilized cells of both strains could completely reduce Cr(VI) in industrial wastewater twice before needing to be replenished. The results suggested that these chromate-reducing bacteria are potential candidates for practical use biotreating industrial effluents containing Cr(VI) with Stenotrophomonas sp. D6 being the more effective bacterium. (C) 2015 Elsevier Ltd. All rights reserved.